Journal article
Effect of specimen type on free immunoglobulin light chains analysis on the Roche Diagnostics cobas 8000 analyzer
SpringerPlus, Vol.4(1), pp.1-9
12/2015
DOI: 10.1186/s40064-015-1546-x
PMCID: PMC4671985
PMID: 26682113
Abstract
The measurement of free immunoglobulin light chains is typically performed on serum; however, the use of alternative specimen types has potential benefits. Using the Freelite™ kappa and lambda free light chains assay on a Roche Diagnostics cobas 8000 c502 analyzer, we compared three specimen types (serum, EDTA-plasma and lithium heparin plasma separator gel-plasma) on 100 patients. Using Deming regression and eliminating outliers (limiting data to light chain concentrations below 400 mg/L), the three specimen types showed comparable results for kappa light chain concentration, lambda light chain concentration, and kappa/lambda ratio with slopes close to 1.0 and y-intercepts close to zero. EDTA-plasma showed slightly more positive bias relative to serum than lithium heparin. Analysis using EDTA-plasma and lithium heparin plasma showed comparable linearity, precision, and temperature stability. A single sample showing hook effect (not in the comparison set) gave comparable results using either plasma specimen type. For the Freelite™ kappa and lambda free light chains assay, both EDTA-plasma or lithium heparin-plasma can serve as acceptable substitutes for serum, at least for the Roche cobas 8000 analyzer.
Details
- Title: Subtitle
- Effect of specimen type on free immunoglobulin light chains analysis on the Roche Diagnostics cobas 8000 analyzer
- Creators
- Louis S Nelson - Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242 USABryan Steussy - Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242 USACory S Morris - Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242 USAMatthew D Krasowski - Department of Pathology, University of Iowa Hospitals and Clinics, Iowa City, IA 52242 USA
- Resource Type
- Journal article
- Publication Details
- SpringerPlus, Vol.4(1), pp.1-9
- DOI
- 10.1186/s40064-015-1546-x
- PMID
- 26682113
- PMCID
- PMC4671985
- NLM abbreviation
- Springerplus
- ISSN
- 2193-1801
- eISSN
- 2193-1801
- Publisher
- Springer International Publishing; Cham
- Language
- English
- Date published
- 12/2015
- Academic Unit
- Pathology
- Record Identifier
- 9984047976602771
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