Journal article
Effect of tempol on peripheral neuropathy in diet-induced obese and high-fat fed/low-dose streptozotocin-treated C57Bl6/J mice
Free radical research, Vol.51(4), pp.360-367
04/03/2017
DOI: 10.1080/10715762.2017.1315767
PMCID: PMC5575770
PMID: 28376643
Abstract
In this study, we sought to determine the efficacy of tempol on multiple neuropathic endpoints in a diet-induced obese mouse, a model of pre-diabetes, and a high-fat fed low-dose streptozotocin treated mouse, a model of type 2 diabetes. Tempol (4-hydroxy-2,2,6,6-tetramethylpiperdine -1-oxyl) is a low molecular weight, water soluble, membrane permeable, and metal-independent superoxide dismutase mimetic that has been widely used in cellular studies for the removal of intracellular and extracellular superoxide. This in vivo study was designed to be an early intervention. Fourteen weeks post-high-fat diet (6 weeks post-hyperglycemia) control, obese, and diabetic mice were divided into no treatment and treatment groups. The treated mice received tempol by gavage (150 mg/kg in water), while the untreated mice received vehicle. The diet-induced obese and the diabetic mice were maintained on the high-fat diet for the duration of the study, while the control group was maintained on the standard diet. Obesity and diabetes caused slowing of motor and sensory nerve conduction, reduction in intraepidermal nerve fiber density, thermal hypoalgesia, and mechanical allodynia. Treatment with tempol partially or completely protected obese and diabetic mice from these deficits. These studies suggest that tempol or other effective scavengers of reactive oxygen species may be a viable option for treating neural complications associated with obesity or type 2 diabetes.
Details
- Title: Subtitle
- Effect of tempol on peripheral neuropathy in diet-induced obese and high-fat fed/low-dose streptozotocin-treated C57Bl6/J mice
- Creators
- Alexander Obrosov - University of IowaHanna Shevalye - University of IowaLawrence J. Coppey - University of IowaMark A. Yorek - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Free radical research, Vol.51(4), pp.360-367
- Publisher
- Taylor & Francis
- DOI
- 10.1080/10715762.2017.1315767
- PMID
- 28376643
- PMCID
- PMC5575770
- ISSN
- 1071-5762
- eISSN
- 1029-2470
- Grant note
- U.S. Department of Veterans Affairs DK107339-02 / National Institute of Diabetes and Digestive and Kidney Diseases NIH Veterans Health Administration DK107339-02 / NIH Blueprint for Neuroscience Research RX000889-05 / Rehabilitation Research and Development Service RX000889-05 / Veterans Health Administration, Office of Research and Development, Rehabilitation Research and Development Office of Research and Development
- Language
- English
- Date published
- 04/03/2017
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine; Ophthalmology and Visual Sciences
- Record Identifier
- 9984359951302771
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