Effect of treatment of diabetic rats with dehydroepiandrosterone on vascular and neural function

Mark A Yorek; Lawrence J Coppey; Jill S Gellett; Eric P Davidson; Xinyu Bing; Donald D Lund; Joseph S Dillon

doi:10.1152/ajpendo.00173.2002

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Effect of treatment of diabetic rats with dehydroepiandrosterone on vascular and neural function

Journal article

Peer reviewed

Effect of treatment of diabetic rats with dehydroepiandrosterone on vascular and neural function

Mark A Yorek, Lawrence J Coppey, Jill S Gellett, Eric P Davidson, Xinyu Bing, Donald D Lund and Joseph S Dillon

American journal of physiology: endocrinology and metabolism, Vol.283(5), pp.E1067-1075

11/2002

DOI: 10.1152/ajpendo.00173.2002

PMID: 12376336

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Abstract

Nutritional supplementation with dehydroepiandrosterone (DHEA) may be a candidate for treating diabetes-induced vascular and neural dysfunction. DHEA is a naturally occurring adrenal androgen that has antioxidant properties and is reportedly reduced in diabetes. Using a prevention protocol, we found that dietary supplementation of streptozotocin-induced diabetic rats with 0.1, 0.25, or 0.5% DHEA caused a concentration-dependent prevention in the development of motor nerve conduction velocity and endoneurial blood flow impairment, which are decreased in diabetes. At 0.25%, DHEA significantly prevented the diabetes-induced increase in serum thiobarbituric acid-reactive substances and sciatic nerve conjugated diene levels. This treatment also reduced the production of superoxide by epineurial arterioles of the sciatic nerve. DHEA treatment (0.25%) significantly improved vascular relaxation mediated by acetylcholine in epineurial vessels of diabetic rats. Sciatic nerve Na+-K+-ATPase activity and myoinositol content was also improved by DHEA treatment, whereas sorbitol and fructose content remained elevated. These studies suggest that DHEA, by preventing oxidative stress and perhaps improving sciatic nerve Na+-K+-ATPase activity, may improve vascular and neural dysfunction in diabetes.

Sciatic Nerve - physiology

Diabetes Mellitus, Experimental - drug therapy

Adjuvants, Immunologic - pharmacology

Dehydroepiandrosterone - pharmacology

Oxidative Stress - physiology

Adjuvants, Immunologic - blood

Body Weight - drug effects

Rats

Blood Glucose

Male

Dehydroepiandrosterone - blood

Diabetic Neuropathies - drug therapy

Peripheral Nerves - drug effects

Arterioles - drug effects

Estrogens - blood

Rats, Sprague-Dawley

Vasoconstriction - drug effects

Animals

Superoxides - metabolism

Sciatic Nerve - drug effects

Diabetes Mellitus, Experimental - physiopathology

Diabetic Neuropathies - physiopathology

Details

Title: Subtitle: Effect of treatment of diabetic rats with dehydroepiandrosterone on vascular and neural function
Creators: Mark A Yorek - Veterans Affairs Medical Center, Diabetes Endocrinology Research Center, and Department of Internal Medicine, University of Iowa, Iowa City, Iowa 52246, USA. myorek@icva.gov
Lawrence J Coppey
Jill S Gellett
Eric P Davidson
Xinyu Bing
Donald D Lund
Joseph S Dillon
Resource Type: Journal article
Publication Details: American journal of physiology: endocrinology and metabolism, Vol.283(5), pp.E1067-1075
DOI: 10.1152/ajpendo.00173.2002
PMID: 12376336
ISSN: 0193-1849
eISSN: 1522-1555
Grant note: R01 AG018928 / NIA NIH HHS DK-58005 / NIDDK NIH HHS DK-25295 / NIDDK NIH HHS AG-18928 / NIA NIH HHS
Language: English
Date published: 11/2002
Academic Unit: Critical Care; Stead Family Department of Pediatrics; Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9984094707202771

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