Journal article
Effect of trifluoperazine on rabbit cortical collecting tubular response to vasopressin
The Journal of physiology, Vol.372(1), pp.41-50
1986
DOI: 10.1113/jphysiol.1986.sp015995
PMCID: PMC1192749
PMID: 3014127
Abstract
Anuran membrane studies suggest that the calcium-binding protein calmodulin is necessary for arginine vasopressin (AVP) to exert a hydro-osmotic effect. We therefore examined the effect of trifluoperazine and N-(6-aminohexyl)-5-chloro-1-naphtholene sulphonamide (W-7), chemically dissimilar calmodulin inhibitors, on hydraulic conductivity (Lp) response to AVP in rabbit cortical collecting tubules perfused in vitro. Trifluoperazine but not W-7 increased basal Lp in rabbit cortical collecting tubules. When cortical collecting tubules were pre-treated with either trifluoperazine or W-7, the effect of AVP to increase Lp was significantly inhibited. To determine the site of this inhibition, Lp responses to exogenous cyclic adenosine 3',5'-phosphate (AMP) were studied. Both trifluoperazine and W-7 pretreatment significantly inhibited the effect of a cyclic AMP analogue to increase rabbit cortical collecting tubule Lp. These results suggest that calmodulin may be an important mediator of the hydro-osmotic response to AVP in the mammalian cortical collecting tube by acting at a site or sites distal to cyclic AMP formation.
Details
- Title: Subtitle
- Effect of trifluoperazine on rabbit cortical collecting tubular response to vasopressin
- Creators
- Mark A Dillingham - Univ. Colorado, health sci. center, Denver CO 80262, United StatesBradley S Dixon - Univ. Colorado, health sci. center, Denver CO 80262, United StatesJin K Kim - Univ. Colorado, health sci. center, Denver CO 80262, United StatesPatricia D Wilson - Univ. Colorado, health sci. center, Denver CO 80262, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of physiology, Vol.372(1), pp.41-50
- Publisher
- Blackwell
- DOI
- 10.1113/jphysiol.1986.sp015995
- PMID
- 3014127
- PMCID
- PMC1192749
- ISSN
- 0022-3751
- eISSN
- 1469-7793
- Language
- English
- Date published
- 1986
- Academic Unit
- Nephrology; Internal Medicine
- Record Identifier
- 9984094741502771
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