Journal article
Effect of unbalanced diets on incorporation of δ-aminolevulinic acid into cytochrome P-450
FEBS letters, Vol.220(1), pp.231-235
1987
DOI: 10.1016/0014-5793(87)80911-0
PMID: 3609316
Abstract
The in vivo syntheses of two liver microsomal cytochromes P-450 PB
3a, P-450 UT
50 [(1987) Eur. J. Biochem., submitted] (
M
r 50 000, 52 000) have been estimated by measuring the specific activity 2 h after i.p. administration of δ-[
3H]aminolevulinic acid to male Sprague Dawley rats. The animals were fed either a standard rat chow (5% lard, 22% casein) or unbalanced diets (high lipid, 30% lard or low protein, 6% casein) with or without 50 ppm Phenoclor DP6. The high-lipid diet supported a more rapid body weight gain but had little impact on cytochrome P-450 content, expressed either per whole liver or per mg microsomal protein, and on the incorporation of the precursor into cytochrome P-450. The latter was determined by measuring the radioactivity incorporated into the cytochrome P-450 fraction, partially purified by afffinity chromatography, as well as into two cytochrome P-450 isozymes (
M
r 50 000 or 52 000) purified by DEAE-52 cellulose ion-exchange chromatography. The low-protein diet, on the other hand, severely depressed body weight gain and cytochrome P-450 content as well as incorporation of radioactivity, the lower-
M
r cytochrome (
M
r 50 000) being particularly affected. However, when a potent inducer, Phenoclor DP6, was added to the low-protein diet, cytochrome synthesis was restored indicating that the effect was reversible.
Details
- Title: Subtitle
- Effect of unbalanced diets on incorporation of δ-aminolevulinic acid into cytochrome P-450
- Creators
- Z Amelizad - Institute for Toxicology, University of Mainz, D-6500 Mainz, FRGJ.F Narbonne - Laboratoire de Toxicologie Alimentaire, Université de Bordeaux I, F-33405 Talence, Bordeaux FranceC Borin - Institut de Physiologie, Université de Toulouse III, F-31400 Toulouse, FranceL.W Robertson - Graduate Center for Toxicology, 204 Funkhouser Bldg, University of Kentucky, Lexington, KY 40506-0054, USAA Periquet - Institut de Physiologie, Université de Toulouse III, F-31400 Toulouse, FranceF Oesch - Institute for Toxicology, University of Mainz, D-6500 Mainz, FRG
- Resource Type
- Journal article
- Publication Details
- FEBS letters, Vol.220(1), pp.231-235
- DOI
- 10.1016/0014-5793(87)80911-0
- PMID
- 3609316
- NLM abbreviation
- FEBS Lett
- ISSN
- 0014-5793
- eISSN
- 1873-3468
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 1987
- Academic Unit
- Occupational and Environmental Health
- Record Identifier
- 9984002580502771
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