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Effect of vitamin C on hyperoxia-induced vasoconstriction in exercising skeletal muscle
Journal article   Open access   Peer reviewed

Effect of vitamin C on hyperoxia-induced vasoconstriction in exercising skeletal muscle

Sushant M Ranadive, Michael J Joyner, Branton G Walker, Jennifer L Taylor and Darren P Casey
Journal of applied physiology (1985), Vol.117(10), pp.1207-1211
11/15/2014
DOI: 10.1152/japplphysiol.00073.2014
PMCID: PMC4233251
PMID: 25237186
url
https://doi.org/10.1152/japplphysiol.00073.2014View
Published (Version of record) Open Access

Abstract

Hyperoxia can cause substantial reductions in peripheral and coronary blood flow at rest and during exercise, which may be caused by reactive oxygen species (ROS) generated during hyperoxia. The aim of this study was to investigate the role of ROS in hyperoxia-induced reductions in skeletal muscle blood flow during forearm exercise. We hypothesized that infusion of vitamin C would abolish the effects of hyperoxia on the forearm blood flow (FBF) responses to exercise. Twelve young healthy adults performed rhythmic forearm handgrip exercise (10% of maximum voluntary contraction for 5 min) during normoxia and hyperoxia. For each condition, two trials were conducted with intra-arterial administration of saline or vitamin C. FBF was measured using Doppler ultrasound. During hyperoxia with saline, FBF and forearm vascular conductance (FVC) were 86.3 ± 5.1 and 86.8 ± 5.2%, respectively, of the normoxic values (100%) (P < 0.05). During vitamin C, hyperoxic FBF and FVC responses were 90.9 ± 4.2 and 90.9 ± 4.1%, respectively, of the normoxic values (P = 0.57 and 0.59). Subjects were then divided into three subgroups based on their percent decrease in FBF (>20, 10-20, and <10%) during hyperoxia. In the subgroup that demonstrated the greatest hyperoxia-induced changes (>20%), FBF and FVC during hyperoxia were 67.1 ± 4.0 and 66.8 ± 3.6%, respectively, of the normoxic values. Vitamin C abolished these effects on FBF and FVC with values that were 102.0 ± 5.2 and 100.8 ± 6.1%, respectively. However, vitamin C had no effect in the other two subgroups. This analysis is consistent with the idea that ROS generation blunts the FBF responses to exercise in the subjects most affected by hyperoxia.
 Hyperoxia - physiopathology Reactive Oxygen Species - metabolism Humans Ascorbic Acid - administration & dosage Male Hyperoxia - diagnostic imaging Regional Blood Flow Vasoconstriction - drug effects Brachial Artery - diagnostic imaging Exercise Forearm Muscle Contraction Time Factors Hyperoxia - metabolism Antioxidants - administration & dosage Blood Flow Velocity Adult Brachial Artery - physiopathology Female Brachial Artery - drug effects Ultrasonography, Doppler, Duplex Hand Strength Infusions, Intra-Arterial Muscle, Skeletal - blood supply

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