Journal article
Effect of wortmannin and 3-AB on G2 phase arrest and transient failure to activate MPF induced by ionizing irradiation
Chinese science bulletin, Vol.43(18), pp.1550-1555
09/1998
DOI: 10.1007/BF02883447
Abstract
Active mitosis promoting factor (MPF) composed of p34odc2 with cyclin B1 is required for the cell cycle transition from G2 to M phase. Recent studies have demonstrated that ionizing radiation (IR) exposures associated with inhibition of p34odc2 activity was the mechanism of G2 arrest. At low dose, IR causes transient failure to dephosphorylate p34odc2 instead of suppression of cyclin B or MPF formation of cyclin B with p34odc2 complex. But the signaling events that regulate p34odc2 in irradiated cells remain unclear. This note demonstrates that PI3 kinase family inhibitor wortmannin (wort) and PARP specific inhibitor 3-AB can reduce the G2 arrest induced by 2Gy γ-ray. Immunoprecipitation studies demonstrate that wort and 3-AB can facilitate dephosphorylation of p34odc2 in G2 phase arrest induced by radiation. These findings suggest that wort sensitive pathway and PARP may be involved in initiating the signal transduction of G2 phase arrest caused by IR.
Details
- Title: Subtitle
- Effect of wortmannin and 3-AB on G2 phase arrest and transient failure to activate MPF induced by ionizing irradiation
- Creators
- Xiangbing Meng - Beijing Institute of Radiation Medicine, Beijing, ChinaZhixian Sun - Beijing Institute of Radiation Medicine, Beijing, China
- Resource Type
- Journal article
- Publication Details
- Chinese science bulletin, Vol.43(18), pp.1550-1555
- Publisher
- Science in China Press
- DOI
- 10.1007/BF02883447
- ISSN
- 1001-6538
- eISSN
- 1861-9541
- Language
- English
- Date published
- 09/1998
- Academic Unit
- Pathology
- Record Identifier
- 9984186486702771
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