Effective blue light photodynamic therapy does not affect cutaneous langerhans cell number or oxidatively damage DNA

Preethi Ramaswamy; Jennifer G Powers; Jag Bhawan; Ildiko Polyak; Barbara A Gilchrest

doi:10.1097/01.DSS.0000452624.01889.8a

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Effective blue light photodynamic therapy does not affect cutaneous langerhans cell number or oxidatively damage DNA

Journal article

Peer reviewed

Effective blue light photodynamic therapy does not affect cutaneous langerhans cell number or oxidatively damage DNA

Preethi Ramaswamy, Jennifer G Powers, Jag Bhawan, Ildiko Polyak and Barbara A Gilchrest

Dermatologic surgery, Vol.40(9), pp.979-987

09/2014

DOI: 10.1097/01.DSS.0000452624.01889.8a

PMID: 25072126

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Abstract

Photodynamic therapy (PDT) using aminolevulinic acid (ALA) with blue light or red light is effective for treating actinic keratoses (AKs). However, immunosuppression follows red light PDT, raising the spectre of skin cancer promotion in treated skin. To determine whether broad-area short incubation (BASI)-ALA-PDT using blue light immunosuppression immunosuppresses treated skin. Patients were evaluated clinically and by standardized facial biopsies of non-AK skin before, 24 hours and 1 month after customary blue light BASI-ALA-PDT. All biopsies were stained for markers of epidermal atypia and Langerhans cells (LCs); and at 24 hours to detect oxidative DNA damage. Patients had an 81% reduction in AKs and slight improvement in clinical and histologic signs of photoaging after 1 month. The biopsied chronically photodamaged skin without clinically detectable AKs showed no effect of PDT on the LC number, distribution, or morphology; and no oxidative DNA damage, in contrast to the changes reported after customary red light PDT. Customary blue light BASI-ALA-PDT does not affect the LC number or produce oxidative DNA damage, the sequelae of red light PDT responsible for immunosuppression in treated skin.

Photochemotherapy - adverse effects

Skin Aging - pathology

Oxidative Stress

Photochemotherapy - methods

Cell Count

Humans

Middle Aged

Skin Aging - drug effects

Aminolevulinic Acid - pharmacology

Color

Skin Aging - radiation effects

Male

Keratinocytes - chemistry

Langerhans Cells - radiation effects

Tumor Suppressor Protein p53 - analysis

Immunosuppression

Photosensitizing Agents - pharmacology

Ki-67 Antigen - analysis

Keratosis, Actinic - drug therapy

Female

Aged

DNA Damage

Keratosis, Actinic - pathology

Langerhans Cells - drug effects

Details

Title: Subtitle: Effective blue light photodynamic therapy does not affect cutaneous langerhans cell number or oxidatively damage DNA
Creators: Preethi Ramaswamy - Department of Dermatology, Boston University School of Medicine, Boston, Massachusetts; †Trokhan Dermatology, Closter, New Jersey; ‡Division of Dermatology, Vanderbilt University School of Medicine, Nashville, Tennessee
Jennifer G Powers
Jag Bhawan
Ildiko Polyak
Barbara A Gilchrest
Resource Type: Journal article
Publication Details: Dermatologic surgery, Vol.40(9), pp.979-987
Publisher: United States
DOI: 10.1097/01.DSS.0000452624.01889.8a
PMID: 25072126
ISSN: 1076-0512
eISSN: 1524-4725
Language: English
Date published: 09/2014
Academic Unit: Dermatology
Record Identifier: 9984025472002771

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