Journal article
Effects of [15N]leucine infused at low rates on leucine metabolism in humans
The American journal of physiology, Vol.249(1 Pt 1), pp.E121-130
07/1985
DOI: 10.1152/ajpendo.1985.249.1.E121
PMID: 4014457
Abstract
The present studies were carried out to determine whether infusions of [15N]leucine at low rates affect estimates of leucine oxidation and of proteolysis and protein synthesis in humans. Three groups of normal subjects were infused for 3 h with either [15N]leucine at a rate of 0.16 or 0.26 mumol X kg-1 X min-1 or saline using [2H3]leucine and alpha-[14C]ketoisocaproate as isotopic tracers of leucine metabolism. Data were analyzed at steady state using both single- and dual-isotope models. Preliminary studies were carried out to characterize the dual-isotope model in humans using infusions of [3H]leucine and alpha-[14C]ketoisocaproate. In the postabsorptive state estimates of leucine appearance, disappearance, and oxidation derived from the two isotope models were in good agreement. Infusion of stable isotope up to approximately 10% of the leucine carbon flux do not have a significant effect on leucine metabolism, but the data derived from such studies must be properly controlled and interpreted with care because these tracers are not massless.
Details
- Title: Subtitle
- Effects of [15N]leucine infused at low rates on leucine metabolism in humans
- Creators
- Paolo TessariEva TsalikianW Frederick SchwenkSteven L NissenMorey W Haymond
- Resource Type
- Journal article
- Publication Details
- The American journal of physiology, Vol.249(1 Pt 1), pp.E121-130
- DOI
- 10.1152/ajpendo.1985.249.1.E121
- PMID
- 4014457
- ISSN
- 0002-9513
- eISSN
- 2163-5773
- Grant note
- AM-26989 / NIADDK NIH HHS AM-07352 / NIADDK NIH HHS RR-585 / NCRR NIH HHS
- Language
- English
- Date published
- 07/1985
- Academic Unit
- Stead Family Department of Pediatrics; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984093605802771
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