Journal article
Effects of 17 beta-estradiol on coronary microvascular responses to endothelin-1
American journal of physiology. Heart and circulatory physiology, Vol.271(3), pp.H1117-H1124
09/01/1996
DOI: 10.1152/ajpheart.1996.271.3.H1117
PMID: 8853349
Abstract
The objective of this study was to examine the effects of 17 beta-estradiol on responses of coronary microvessels to endothelin-1 (ET-1). With the use of isolated pressurized coronary microvessels from the left ventricle of male or female dogs, constrictions to ET-1 were similar in vessels from male and female dogs. 17 beta-Estradiol (1 microM) attenuated constriction to ET-1 of small arteries from both male (percent constriction at 10 microM control: 39 +/- 9%, estradiol: 3 +/- 2%; P < 0.05) and female (percent constriction at 10 microM control: 39 +/- 8%, estradiol: 6 +/- 3%; P < 0.05) dogs similarly. In contrast, testosterone (1 microM) had no effect on constriction to ET-1. Constrictions to ET-1 were completely abolished by BQ-123 (1 microM), a selective ETA-receptor antagonist, and enhanced by BQ-788 (1 microM), a selective ETB-receptor antagonist. Constrictions to ET-1 alone were not altered by indomethacin (Indo, 10 microM) or NG-nitro-L-arginine (L-NNA, 100 microM). 17 beta-Estradiol produced dose-dependent relaxation of coronary microvessels preconstricted with ET-1 that was similar to the response to testosterone and progesterone. Indo or L-NNA alone had no effect on relaxation to 17 beta-estradiol. However, the combination of Indo and L-NNA attenuated Taxation to 17 beta-estradiol (percent dilation at 1 microM control: 64 +/- 13%; Indo plus L-NNA: 21 +/- 6%; P < 0.05) but did not affect relaxation to testosterone. Thus 17 beta-estradiol attenuated constrictions of coronary microvessels to ET-1 more than did similar concentrations of testosterone. The ability of 17 beta-estradiol to modulate responses to endothelin may involve release of vasodilator prostaglandins and/or nitric oxide by 17 beta-estradiol.
Details
- Title: Subtitle
- Effects of 17 beta-estradiol on coronary microvascular responses to endothelin-1
- Creators
- Kathryn G Lamping - Department of Internal Medicine, College of Medicine, University ofIowa, Iowa City 52242, USADaniel W Nuno - Department of Internal Medicine, College of Medicine, University ofIowa, Iowa City 52242, USA
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.271(3), pp.H1117-H1124
- DOI
- 10.1152/ajpheart.1996.271.3.H1117
- PMID
- 8853349
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Language
- English
- Date published
- 09/01/1996
- Academic Unit
- Cardiovascular Medicine; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984094875302771
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