Journal article
Effects of 28-Day Nose-Only Inhalation of PCB52 (2,2',5,5'-Tetrachlorobiphenyl) on the Brain Transcriptome
Toxicology (Amsterdam), Vol.509, 153965
10/04/2024
DOI: 10.1016/j.tox.2024.153965
PMCID: PMC11588532
PMID: 39369937
Abstract
A semi-volatile polychlorinated biphenyl (PCB) congener, PCB52, is present in the indoor air of schools; however, the effects of inhaled PCB52 on the brain have not been investigated. This study exposed male Sprague-Dawley rats at 39 days of age and female rats at 42 days of age to PCB52 for 4hours per day over 28 consecutive days through nose-only inhalation. Neurobehavioral tests were conducted during the last 5 days of exposure. The total estimated PCB52 exposures after 28 days were 1080±20µg/kg BW for male rats and 1140±10µg/kg BW for female rats. PCB52 and its metabolites were detected by gas chromatography-tandem mass spectrometry in the brain, lung, and serum, with the lung showing the highest concentrations. PCB52 levels were higher in the brains of females than males. Males showed increased exploratory behavior compared to controls, whereas females exhibited decreased exploratory behavior compared to controls in the same tests. PCB52 exposure did not impact locomotor activity or working memory. Gene expression and pathway analysis in the striatum and cerebellum suggest that PCB52 inhalation causes mitochondrial dysfunction. No significant differences were observed by immunohistochemical evaluation in the density and percent area of total cells, astrocytes, or microglia in the striatum and cerebellar cortex. Our results indicate multilevel effects of inhaled PCB52 on the rat brain, from gene expression to behavioral effects.
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•PCB52 and its metabolites were detected in the rat brain•PCB52-exposed males showed increased exploratory behavior compared to controls•PCB52-exposed females exhibited decreased exploratory behavior compared to controls•PCB52 inhalation caused brain region-specific transcriptomic changes•The “oxidative phosphorylation” pathway was affected by PCB52 inhalation
Details
- Title: Subtitle
- Effects of 28-Day Nose-Only Inhalation of PCB52 (2,2',5,5'-Tetrachlorobiphenyl) on the Brain Transcriptome
- Creators
- Amanda J. Bullert - University of IowaHui Wang - University of Iowa, Occupational and Environmental HealthMorgan J. Linahon - University of IowaMichael S. Chimenti - University of IowaAndrea Adamcakova-Dodd - University of IowaXueshu Li - University of IowaMichael E. Dailey - University of IowaAloysius J. Klingelhutz - University of IowaJames A. Ankrum - University of IowaHanna E. Stevens - University of IowaPeter S. Thorne - University of IowaHans-Joachim Lehmler - Department of Occupational and Environmental Health, The University of Iowa, Iowa City, Iowa, USA
- Resource Type
- Journal article
- Publication Details
- Toxicology (Amsterdam), Vol.509, 153965
- DOI
- 10.1016/j.tox.2024.153965
- PMID
- 39369937
- PMCID
- PMC11588532
- NLM abbreviation
- Toxicology
- ISSN
- 0300-483X
- eISSN
- 1879-3185
- Publisher
- Elsevier B.V
- Grant note
- Iowa Superfund Research Pro-gram: NIH P42 ES013661, NIH P30 ES005605
This research was supported by the Iowa Superfund Research Pro-gram (NIH P42 ES013661) and conducted in laboratory facilities of the Environmental Health Sciences Research Center (NIH P30 ES005605) . The content is solely the responsibility of the authors and does not necessarily represent the official views of the funding agencies listed above.
- Language
- English
- Date published
- 10/04/2024
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Psychiatry; Civil and Environmental Engineering; Microbiology and Immunology; Occupational and Environmental Health; Iowa Neuroscience Institute; Biology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Iowa Superfund Research Program; Iowa Institute of Human Genetics
- Record Identifier
- 9984721442402771
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