Journal article
Effects of Age and Diet on Triglyceride Metabolism in Mice
Journal of lipid research, Vol.66(1), 100706
01/2025
DOI: 10.1016/j.jlr.2024.100706
PMCID: PMC11730548
PMID: 39566846
Abstract
Both age and diet can contribute to alterations in triglyceride metabolism and subsequent metabolic disease. In humans, plasma triglyceride levels increase with age. Diets high in saturated fats can increase triglyceride levels while diets high in omega-3 fatty acids decrease triglyceride levels. Here we asked how age and long-term diet altered triglyceride metabolism in mice. We fed male and female C57Bl/6 mice a low-fat diet, a western diet (WD), or a diet high in polyunsaturated and omega-3 fatty acids (n3D) for up to 2 years. We measured survival, body composition, plasma triglyceride levels, chylomicron clearance, and oral fat, glucose, and insulin tolerance. Triglyceride levels in mice did not increase with age, regardless of diet. Oral fat tolerance increased with age, while chylomicron clearance remained unchanged. Decreased survival was observed in WD-fed mice. Interestingly, n3D-fed mice diet gained more lean mass and had lower insulin levels than WD-fed or LFD-fed mice. Moreover, triglyceride uptake into the hearts of n3D-fed mice was strikingly higher than in other groups. Our data indicate that in C57Bl/6 mice, age-induced changes in triglyceride metabolism differ from those observed in humans. Mice, like humans, appeared to have decreased fat absorption with age, but in mice plasma triglyceride clearance did not decrease with age, resulting in lower plasma triglyceride levels and improved fat tolerance with age. Although a chronic diet high in omega-3 fatty acids increased insulin sensitivity and triglyceride uptake specifically into the heart, how these observations are connected is unclear.
Details
- Title: Subtitle
- Effects of Age and Diet on Triglyceride Metabolism in Mice
- Creators
- Kathryn M. Spitler - University of IowaShwetha K. Shetty - University of IowaBrandon S.J. Davies - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of lipid research, Vol.66(1), 100706
- DOI
- 10.1016/j.jlr.2024.100706
- PMID
- 39566846
- PMCID
- PMC11730548
- NLM abbreviation
- J Lipid Res
- ISSN
- 0022-2275
- eISSN
- 1539-7262
- Publisher
- ELSEVIER
- Grant note
- NIH: R01 HL130146 University of Iowa Aging Initiative Pilot Grant
NIH R01 HL130146 to B. S. J. D. and a University of Iowa Aging Initiative Pilot Grant to B. S. J. D.
- Language
- English
- Electronic publication date
- 11/2024
- Date published
- 01/2025
- Academic Unit
- Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology
- Record Identifier
- 9984749256202771
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