Journal article
Effects of Charged Cluster Mutations on the Function of Herpes Simplex Virus Type 1 UL34 Protein
Journal of virology, Vol.77(13), pp.7601-7610
07/2003
DOI: 10.1128/JVI.77.13.7601-7610.2003
PMCID: PMC164785
PMID: 12805460
Abstract
Herpes simplex virus type 1 (HSV-1) is a DNA virus that acquires an envelope by budding into the inner nuclear membrane of an infected cell. Recombinant HSV-1 lacking the U
L
34 gene cannot undergo this event. U
L
34 and U
L
31, another viral protein, colocalize in an infected cell and are necessary and sufficient to target both proteins to the inner nuclear envelope. In order to define and characterize sequences of U
L
34 that are necessary for primary envelopment to occur, a library of 19 U
L
34 charged cluster mutants and a truncation mutant lacking the putative transmembrane domain (ΔTM) were generated. Mutants in this library were analyzed in a complementation assay for their ability to function in the production of infectious virus. Seven of the mutants failed to complement a U
L
34-null virus. The remainder of the mutants complemented at or near wild-type U
L
34 levels. Failure of a mutant protein to function might be the result of incorrect subcellular localization. To address this possibility, confocal microscopy was used to determine the localization of the U
L
34 protein in charged cluster mutants and ΔTM. In transfection-infection experiments, all of the functional U
L
34 mutants and four of the six noncomplementing mutants localized to the inner nuclear envelope in a manner indistinguishable from that of wild-type U
L
34. All of the noncomplementing U
L
34 mutants mediated proper localization of U
L
31. Charged clusters critical for U
L
34 function are dispersed throughout the protein sequence and do not correlate well with highly conserved regions of the protein. These data suggest that U
L
34 has at least one function in addition to mediating proper localization of U
L
31 in infected cells and provide further support for the role of U
L
34 in mediating proper localization of U
L
31 in infected cells.
Details
- Title: Subtitle
- Effects of Charged Cluster Mutations on the Function of Herpes Simplex Virus Type 1 UL34 Protein
- Creators
- Susan L Bjerke - Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853John M Cowan - Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853Jelani K Kerr - Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853Ashley E Reynolds - Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853Joel D Baines - Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853Richard J Roller - Department of Microbiology and Immunology, Cornell University, Ithaca, New York 14853
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.77(13), pp.7601-7610
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/JVI.77.13.7601-7610.2003
- PMID
- 12805460
- PMCID
- PMC164785
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Language
- English
- Date published
- 07/2003
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001119602771
Metrics
20 Record Views