Effects of Dietary Fish Oil on the Depletion of Carcinogenic PAH-DNA Adduct Levels in the Liver of B6C3F1 Mouse

Guo-Dong Zhou; Huiping Zhu; Tracie D. Phillips; Jianbo Wang; Shi-Zhou Wang; Fen Wang; Brad A. Amendt; Xanthi I. Couroucli; Kirby C. Donnelly; Bhagavatula Moorthy

doi:10.1371/journal.pone.0026589

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Effects of Dietary Fish Oil on the Depletion of Carcinogenic PAH-DNA Adduct Levels in the Liver of B6C3F1 Mouse

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Effects of Dietary Fish Oil on the Depletion of Carcinogenic PAH-DNA Adduct Levels in the Liver of B6C3F1 Mouse

Guo-Dong Zhou, Huiping Zhu, Tracie D. Phillips, Jianbo Wang, Shi-Zhou Wang, Fen Wang, Brad A. Amendt, Xanthi I. Couroucli, Kirby C. Donnelly and Bhagavatula Moorthy

PloS one, Vol.6(10), pp.e26589-e26589

10/31/2011

DOI: 10.1371/journal.pone.0026589

PMCID: PMC3204992

PMID: 22066002

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Abstract

Many carcinogenic polycyclic aromatic hydrocarbons (PAHs) and their metabolites can bind covalently to DNA. Carcinogen-DNA adducts may lead to mutations in critical genes, eventually leading to cancer. In this study we report that fish oil (FO) blocks the formation of DNA adducts by detoxification of PAHs. B6C3F1 male mice were fed a FO or corn oil (CO) diet for 30 days. The animals were then treated with seven carcinogenic PAHs including benzo(a)pyrene (BaP) with one of two doses via a single intraperitoneal injection. Animals were terminated at 1, 3, or 7 d after treatment. The levels of DNA adducts were analyzed by the 32 P-postlabeling assay. Our results showed that the levels of total hepatic DNA adducts were significantly decreased in FO groups compared to CO groups with an exception of low PAH dose at 3 d (P = 0.067). Total adduct levels in the high dose PAH groups were 41.36±6.48 (Mean±SEM) and 78.72±8.03 in 10 9 nucleotides (P = 0.011), respectively, for the FO and CO groups at 7 d. Animals treated with the low dose (2.5 fold lower) PAHs displayed similar trends. Total adduct levels were 12.21±2.33 in the FO group and 24.07±1.99 in the CO group, P = 0.008. BPDE-dG adduct values at 7 d after treatment of high dose PAHs were 32.34±1.94 (CO group) and 21.82±3.37 (FO group) in 10 9 nucleotides with P value being 0.035. Low dose groups showed similar trends for BPDE-dG adduct in the two diet groups. FO significantly enhanced gene expression of Cyp1a1 in both the high and low dose PAH groups. Gstt1 at low dose of PAHs showed high levels in FO compared to CO groups with P values being 0.014. Histological observations indicated that FO played a hepatoprotective role during the early stages. Our results suggest that FO has a potential to be developed as a cancer chemopreventive agent.

Biology

Medicine

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Title: Subtitle: Effects of Dietary Fish Oil on the Depletion of Carcinogenic PAH-DNA Adduct Levels in the Liver of B6C3F1 Mouse
Creators: Guo-Dong Zhou - IIT Research Institute
Huiping Zhu - The University of Texas at Austin
Tracie D. Phillips - Texas A&M University System
Jianbo Wang - Texas A&M University System
Shi-Zhou Wang - Texas A&M University System
Fen Wang - Texas A&M University System
Brad A. Amendt - Texas A&M University System
Xanthi I. Couroucli - Baylor College of Medicine
Kirby C. Donnelly - Texas A&M University System
Bhagavatula Moorthy - Baylor College of Medicine
Resource Type: Journal article
Publication Details: PloS one, Vol.6(10), pp.e26589-e26589
DOI: 10.1371/journal.pone.0026589
PMID: 22066002
PMCID: PMC3204992
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science
Alternative title: Dietary Fish Oil Decreases PAH-DNA Adduct Levels
Language: English
Date published: 10/31/2011
Academic Unit: Orthodontics; Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Dental Research
Record Identifier: 9984284454202771

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