Journal article
Effects of Episodic Alcohol Exposure on BMP2 Signaling During Tibia Fracture Healing
Journal of orthopaedic trauma, Vol.32(6), pp.288-295
06/01/2018
DOI: 10.1097/BOT.0000000000001160
PMCID: PMC7485276
PMID: 29672339
Abstract
Objectives: To explore how alcohol affects the BMP-2 signaling pathway, which is known to play a critical role in bone and cartilage formation during fracture healing.
Methods: A rat model was used to demonstrate the detrimental effects of alcohol exposure on tibia fracture healing. Specific components of the BMP-2 pathway were analyzed in fracture callus on days 3, 7, 14, and 21 after fracture via western immunoassays and enzyme-linked immunosorbent assay.
Results: Alcohol exposure before tibia fracture demonstrated attenuation of downstream BMP-2 signaling. The BMP-2 antagonist, Chordin, may be the central component of the BMP-2-related changes demonstrated in this study. Although alcohol affected BMP-related proteins at all time points, it seems that day 14 after fracture is a critical time point for alcohol-related modulation of callus formation in our model.
Conclusions: This study may provide the scientific basis for further studies addressing whether the application of exogenous BMP-2 in patients with a history of alcohol abuse who sustain long bone fractures may or may not be of benefit.
Details
- Title: Subtitle
- Effects of Episodic Alcohol Exposure on BMP2 Signaling During Tibia Fracture Healing
- Creators
- Anthony Bratton - Loyola University ChicagoJoshua Eisenberg - Loyola University ChicagoAleksandra Vuchkovska - Loyola University ChicagoPhilip Roper - Loyola University ChicagoJohn J. Callaci - Loyola University Chicago
- Resource Type
- Journal article
- Publication Details
- Journal of orthopaedic trauma, Vol.32(6), pp.288-295
- DOI
- 10.1097/BOT.0000000000001160
- PMID
- 29672339
- PMCID
- PMC7485276
- NLM abbreviation
- J Orthop Trauma
- ISSN
- 0890-5339
- eISSN
- 1531-2291
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 8
- Grant note
- Orthopaedic Trauma Association R21AA021225 / NIH/NIAAA; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Alcohol Abuse & Alcoholism (NIAAA) R21AA025551 / NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute on Alcohol Abuse & Alcoholism (NIAAA)
- Language
- English
- Date published
- 06/01/2018
- Academic Unit
- Orthopedics and Rehabilitation
- Record Identifier
- 9984702944702771
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