Effects of Fluorescein Staining on Laser In Vivo Confocal Microscopy Images of the Cornea

Christine W Sindt; D. Brice Critser; Trudy K Grout; Jami R Kern

doi:10.1155/2012/541974

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Effects of Fluorescein Staining on Laser In Vivo Confocal Microscopy Images of the Cornea

Journal article

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Effects of Fluorescein Staining on Laser In Vivo Confocal Microscopy Images of the Cornea

Christine W Sindt, D. Brice Critser, Trudy K Grout and Jami R Kern

Journal of Ophthalmology, Vol.2012, pp.541974-4

2012

DOI: 10.1155/2012/541974

PMCID: PMC3272843

PMID: 22363837

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https://doi.org/10.1155/2012/541974View

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Abstract

This study was designed to identify whether topical fluorescein, a common ophthalmic tool, affects laser in vivo confocal microscopy of the cornea, a tool with growing applications. Twenty-five eye care specialists were asked to identify presence or absence of fluorescein in 99 confocal micrographs of healthy corneas. Responses were statistically similar to guessing for the epithelium (48% ± 14% of respondents correct per image) and the subbasal nerve plexus (49% ± 11% correct), but results were less clear for the stroma. Dendritic immune cells were quantified in bilateral images from subjects who had been unilaterally stained with fluorescein. Density of dendritic immune cells was statistically similar between the unstained and contralateral stained eyes of 24 contact lens wearers ( P = .72) and of 10 nonwearers ( P = .53). Overall, the results indicated that fluorescein staining did not interfere with laser confocal microscopy of corneal epithelium, subbasal nerves, or dendritic immune cells.

Clinical Study

Details

Title: Subtitle: Effects of Fluorescein Staining on Laser In Vivo Confocal Microscopy Images of the Cornea
Creators: Christine W Sindt - Alcon Research Ltd., 6201 South Freeway, Fort Worth, TX 76134, USA
D. Brice Critser - Alcon Research Ltd., 6201 South Freeway, Fort Worth, TX 76134, USA
Trudy K Grout - Alcon Research Ltd., 6201 South Freeway, Fort Worth, TX 76134, USA
Jami R Kern - Alcon Research Ltd., 6201 South Freeway, Fort Worth, TX 76134, USA
Resource Type: Journal article
Publication Details: Journal of Ophthalmology, Vol.2012, pp.541974-4
DOI: 10.1155/2012/541974
PMID: 22363837
PMCID: PMC3272843
NLM abbreviation: J Ophthalmol
ISSN: 2090-004X
eISSN: 2090-0058
Publisher: Hindawi Publishing Corporation
Grant note: DOI: 10.13039/100007816, name: Alcon
Language: English
Date published: 2012
Academic Unit: The University of Iowa Institute for Vision Research; Ophthalmology and Visual Sciences
Record Identifier: 9983979995902771

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