Effects of Polychlorinated Biphenyls (PCBs) and Their Hydroxylated Metabolites (OH-PCBs) on Arabidopsis thaliana

Srishty Subramanian; Jerald L Schnoor; Benoit Van Aken

doi:10.1021/acs.est.7b01538

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Effects of Polychlorinated Biphenyls (PCBs) and Their Hydroxylated Metabolites (OH-PCBs) on Arabidopsis thaliana

Journal article

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Effects of Polychlorinated Biphenyls (PCBs) and Their Hydroxylated Metabolites (OH-PCBs) on Arabidopsis thaliana

Srishty Subramanian, Jerald L Schnoor and Benoit Van Aken

Environmental science & technology, Vol.51(12), pp.7263-7270

06/20/2017

DOI: 10.1021/acs.est.7b01538

PMCID: PMC5772893

PMID: 28541669

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https://www.ncbi.nlm.nih.gov/pmc/articles/5772893View

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Abstract

Plants metabolize polychlorinated biphenyls (PCBs) into hydroxylated derivatives (OH-PCBs), which are sometimes more toxic than the parent PCBs. The objective of this research was to compare the toxicity of a suite of PCBs and OH-PCBs toward the model plant, Arabidopsis thaliana. While parent PCBs and higher-chlorinated OH-PCBs exhibited a low or nondetectable toxicity, lower-chlorinated OH-PCBs significantly inhibited the germination rate and plant growth, with inhibition concentration 50% (IC ) ranging from 1.6 to 12.0 mg L . The transcriptomic response of A. thaliana to 2,5-dichlorobiphenyl (2,5-DCB), and its OH metabolite, 4'-OH-2,5-DCB, was then examined using whole-genome expression microarrays (Affymetrix). Exposure to 2,5-DCB and 4'-OH-2,5-DCB resulted in different expression patterns, with the former leading to enrichment of genes involved in response to toxic stress and detoxification functions. Exposure to 2,5-DCB induced multiple xenobiotic response genes, such as cytochrome P-450 and glutathione S-transferases, potentially involved in the PCB metabolism. On the contrary, exposure to both compounds resulted in the down-regulation of genes involved in stresses not directly related to toxicity. Unlike its OH derivative, 2,5-DCB was shown to induce a transcriptomic profile similar to plant safeners, which are nontoxic chemicals stimulating detoxification pathways in plants. The differentiated induction of detoxification enzymes by 2,5-DCB may explain its lower phytotoxicity compared to 4'-OH-2,5-DCB.

Gene Expression Regulation - drug effects

Hydroxylation

Polychlorinated Biphenyls - toxicity

Arabidopsis

Down-Regulation

Cytochrome P-450 Enzyme System - metabolism

Environmental Pollutants - toxicity

ISRP Project 5 2015-2020

Synthesis Core

Details

Title: Subtitle: Effects of Polychlorinated Biphenyls (PCBs) and Their Hydroxylated Metabolites (OH-PCBs) on Arabidopsis thaliana
Creators: Srishty Subramanian - Department of Civil & Environmental Engineering, Temple University , Philadelphia, Pennsylvania 19122, United States
Jerald L Schnoor - Department of Civil & Environmental Engineering, The University of Iowa , Iowa City, Iowa 52242, United States
Benoit Van Aken - Department of Civil & Environmental Engineering, Temple University , Philadelphia, Pennsylvania 19122, United States
Resource Type: Journal article
Publication Details: Environmental science & technology, Vol.51(12), pp.7263-7270
DOI: 10.1021/acs.est.7b01538
PMID: 28541669
PMCID: PMC5772893
NLM abbreviation: Environ Sci Technol
ISSN: 0013-936X
eISSN: 1520-5851
Publisher: United States
Grant note: P42 ES013661 / NIEHS NIH HHS P30 ES005605 / NIEHS NIH HHS
Language: English
Date published: 06/20/2017
Academic Unit: Civil and Environmental Engineering; Occupational and Environmental Health; Iowa Superfund Research Program
Record Identifier: 9983997982902771

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