Journal article
Effects of Tham Nasal Alkalinization on Airway Microbial Communities: A Pilot Study in Non-CF and CF Adults
Annals of otology, rhinology & laryngology, Vol.131(9), pp.1013-1020
10/21/2021
DOI: 10.1177/00034894211051814
PMCID: PMC9021322
PMID: 34674574
Abstract
Objectives: In cystic fibrosis (CF), loss of CFTR-mediated bicarbonate secretion reduces the airway surface liquid (ASL) pH causing airway host defense defects. Aerosolized sodium bicarbonate can reverse these defects, but its effects are short-lived. Aerosolized tromethamine (THAM) also raises the ASL pH but its effects are much longer lasting. In this pilot study, we tested the hypothesis that nasally administered THAM would alter the nasal bacterial composition in adults with and without CF.
Methods: Subjects (n = 32 total) received intranasally administered normal saline or THAM followed by a wash out period prior to receiving the other treatment. Nasal bacterial cultures were obtained prior to and after each treatment period.
Results: At baseline, nasal swab bacterial counts were similar between non-CF and CF subjects, but CF subjects had reduced microbial diversity. Both nasal saline and THAM were well-tolerated. In non-CF subjects, nasal airway alkalinization decreased both the total bacterial density and the gram-positive bacterial species recovered. In both non-CF and CF subjects, THAM decreased the amount of Corynebacterium accolens detected, but increased the amount of Corynebacterium pseudodiphtheriticum recovered on nasal swabs. A reduction in Staphylococcus aureus nasal colonization was also found in subjects who grew C. pseudodiphtheriticum.
Conclusions: This study shows that aerosolized THAM is safe and well-tolerated and that nasal airway alkalinization alters the composition of mucosal bacterial communities.
Details
- Title: Subtitle
- Effects of Tham Nasal Alkalinization on Airway Microbial Communities: A Pilot Study in Non-CF and CF Adults
- Creators
- Zachary M. Holliday - Roy J. and Lucille A. Carver College of MedicineJanice L. Launspach - Roy J. and Lucille A. Carver College of MedicineLakshmi Durairaj - Roy J. and Lucille A. Carver College of MedicinePradeep K. Singh - University of WashingtonJoseph Zabner - Roy J. and Lucille A. Carver College of MedicineDavid A. Stoltz - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Annals of otology, rhinology & laryngology, Vol.131(9), pp.1013-1020
- DOI
- 10.1177/00034894211051814
- PMID
- 34674574
- PMCID
- PMC9021322
- NLM abbreviation
- Ann Otol Rhinol Laryngol
- ISSN
- 0003-4894
- eISSN
- 1943-572X
- Publisher
- Sage
- Number of pages
- 8
- Grant note
- HL136813; HL091842; HL007638 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA UL1TR002537 / National Center For Advancing Translational Sciences of the National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Advancing Translational Sciences (NCATS) Cystic Fibrosis Foundation (Iowa Cystic Fibrosis Foundation Research Development Program)
- Language
- English
- Date published
- 10/21/2021
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984297503402771
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