Effects of Total Alkaloids of Sophora alopecuroides on Biofilm Formation in Staphylococcus epidermidis

Xue Li; Cuiping Guan; Yulong He; Yujiong Wang; Xiaoming Liu; Xuezhang Zhou

doi:10.1155/2016/4020715

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Effects of Total Alkaloids of Sophora alopecuroides on Biofilm Formation in Staphylococcus epidermidis

Journal article

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Effects of Total Alkaloids of Sophora alopecuroides on Biofilm Formation in Staphylococcus epidermidis

Xue Li, Cuiping Guan, Yulong He, Yujiong Wang, Xiaoming Liu and Xuezhang Zhou

BioMed research international, Vol.2016, pp.1-10

06/19/2016

DOI: 10.1155/2016/4020715

PMCID: PMC4930803

PMID: 27413745

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Abstract

Staphylococcus epidermidis (S. epidermidis) is an opportunistic pathogen with low pathogenicity and a cause of the repeated outbreak of bovine mastitis in veterinary clinical settings. In this report, a biofilm model of S. epidermidis was generated and the minimal inhibitory concentration (MIC) and sub-MIC (SMIC) on bacterial cultures were assessed for the following agents: total alkaloids of Sophora alopecuroides (TASA), ciprofloxacin (CIP), and erythromycin (ERY). The formation and characteristic parameters of biofilm were analyzed in terms of XTT assay, silver staining, and confocal laser scanning microscope (CLSM). Results showed that a sub-MIC of TASA could inhibit 50% biofilm of bacterial activity, while 250-fold MIC of CIP and ERY MICs only inhibited 50% and 47% of biofilm formation, respectively. All three agents could inhibit the biofilm formation at an early stage, but TASA showed a better inhibitory effect on the late stage of biofilm thickening. A morphological analysis using CLSM further confirmed the destruction of biofilm by these agents. These results thus suggest that TASA has an inhibitory effect on biofilm formation of clinic S. epidermidis, which may be a potential agent warranted for further study on the treatment prevention of infection related to S. epidermidis in veterinary clinic.

Details

Title: Subtitle: Effects of Total Alkaloids of Sophora alopecuroides on Biofilm Formation in Staphylococcus epidermidis
Creators: Xue Li - Key Laboratory of the Ministry of Education for the Conservation and Utilization of Special Biological Resources of Western China Yinchuan Ningxia 750021 China
Cuiping Guan - Ningxia University
Yulong He - Ningxia University
Yujiong Wang - Ningxia University
Xiaoming Liu - Ningxia University
Xuezhang Zhou - Ningxia University
Contributors: Carla R. Arciola (Editor)
Resource Type: Journal article
Publication Details: BioMed research international, Vol.2016, pp.1-10
DOI: 10.1155/2016/4020715
PMID: 27413745
PMCID: PMC4930803
NLM abbreviation: Biomed Res Int
ISSN: 2314-6133
eISSN: 2314-6141
Publisher: Hindawi Publishing Corporation
Grant note: DOI: 10.13039/501100001809, name: National Natural Science Foundation of China, award: 31260624, 31060348
Language: English
Date published: 06/19/2016
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984288570202771

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