Journal article
Effects of antioxidant enzyme overexpression on the invasive phenotype of hamster cheek pouch carcinoma cells
Free radical biology & medicine, Vol.27(5), pp.572-579
1999
DOI: 10.1016/S0891-5849(99)00109-4
PMID: 10490277
Abstract
To examine the role of reactive oxygen species on the invasive phenotype of cancer cells, we overexpressed manganese- and copper-zinc-containing superoxide dismutases (MnSOD, CuZnSOD) and catalase (Cat) in hamster cheek pouch carcinoma (HCPC-1) cells in vitro using adenoviral vector-mediated gene transfer. Hamster cheek pouch carcinoma cells were transduced with these adenoviral vector constructs alone, or in combination, at concentrations [i.e., multiplicity of infectivity (MOI)] of 100 MOI each. The
Escherichia coli β-
galactosidase reporter construct was used as a control virus. Protein expression was examined by Western blot analysis and enzymatic activities were measured using spectrophotometry. To observe the effects of transgene overexpression on in vitro tumor cell invasion, we used the membrane invasion culture system, an accurate and reliable method for examining tumor cell invasion, in vitro. This assay measures the ability of tumor cells to invade a basement membrane matrix consisting of type IV collagen, laminin, and gelatin. MnSOD overexpression resulted in a 50% increase in HCPC-1 cell invasiveness (
p < .001); co-overexpression of MnSOD with Cat partially inhibited this effect (
p < .05). Moreover, co-overexpression of both SODs resulted in a significant increase in invasiveness compared with the parental HCPC-1 cells (
p < .05). These changes could not be correlated with the 72 kDa collagenase IV or stromolysin activities using zymography, or the downregulation of the adhesion molecules E-cadherin or the α4 subunit of the α4β1 integrin. These results suggest that hydrogen peroxide may play a role in the process of tumor cell invasion, but that the process does not rely on changes in matrix metalloproteinase activity in the cells, or the expression of cell adhesion molecules.
Details
- Title: Subtitle
- Effects of antioxidant enzyme overexpression on the invasive phenotype of hamster cheek pouch carcinoma cells
- Creators
- Ernest W.N Lam - Department of Radiology, University of Iowa, Iowa City, IA, USARalf Zwacka - Human Genetics Unit, Western General Hospital, Edinburgh, United KingdomElizabeth A Seftor - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA, USADaniel R.C Nieva - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA, USABeverly L Davidson - Department of Internal Medicine, University of Iowa, Iowa City, IA, USAJohn F Engelhardt - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA, USAMary J.C Hendrix - Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA, USALarry W Oberley - Department of Radiology, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.27(5), pp.572-579
- DOI
- 10.1016/S0891-5849(99)00109-4
- PMID
- 10490277
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 1999
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025677002771
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