Journal article
Effects of calcitonin gene-related peptide on opossum esophageal smooth muscle
Gastroenterology (New York, N.Y. 1943), Vol.113(2), pp.514-520
1997
DOI: 10.1053/gast.1997.v113.pm9247471
PMID: 9247471
Abstract
BACKGROUND & AIMS: Peristaltic contraction of the esophagus and relaxation of the lower esophageal sphincter (LES) are mediated by nitric oxide (NO.)-producing myenteric nerves. NO synthase immunoreactivity and calcitonin gene-related peptide (CGRP) immunoreactivity colocalize in these nerves. CGRP relaxes the LES and decreases the amplitude of esophageal peristalsis. The aim of this study was to test the hypothesis that CGRP plays a role in nerve- induced responses of the esophagus.
METHODS: Smooth muscle strips from the LES and the body of the esophagus were stimulated by an electrical field to evoke nerve-mediated responses.
RESULTS: CGRP decreased the amplitude and lengthened the latency of the off-response, and it relaxed the LES. Tetrodotoxin did not block the CGRP-induced LES relaxation. The CGRP antagonist CGRP 8-37 inhibited the effects of exogenous CGRP. It increased the amplitude and shortened the latency of the off-response, and it increased the resting tone of the LES. CGRP 8- 37 did not inhibit LES relaxation or esophageal contraction. N omega- nitro-L-arginine abolished nerve-induced relaxation of the LES muscle and the esophageal off-response but not the relaxation produced by CGRP.
CONCLUSIONS: These data support the hypothesis that CGRP may decrease the LES basal tone and modulate the timing and amplitude of the esophageal off-responses.
Details
- Title: Subtitle
- Effects of calcitonin gene-related peptide on opossum esophageal smooth muscle
- Creators
- Aliye Uc - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa, United StatesJoseph A Murray - Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa, United StatesJeffrey L Conklin - Department of Pediatrics, University of Iowa College of Medicine, Iowa City, Iowa, United States
- Resource Type
- Journal article
- Publication Details
- Gastroenterology (New York, N.Y. 1943), Vol.113(2), pp.514-520
- DOI
- 10.1053/gast.1997.v113.pm9247471
- PMID
- 9247471
- NLM abbreviation
- Gastroenterology
- ISSN
- 0016-5085
- eISSN
- 1528-0012
- Publisher
- Elsevier; New York, NY
- Language
- English
- Date published
- 1997
- Academic Unit
- Stead Family Department of Pediatrics; Radiation Oncology; Gastroenterology, Hepatology, Pancreatology, and Nutrition
- Record Identifier
- 9984047894402771
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