Effects of chronic nitric oxide synthase inhibition on cerebral arterioles in Wistar-Kyoto rats

Jean-Marc Chillon; Gary L Baumbach

doi:10.1097/00004872-200403000-00015

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Effects of chronic nitric oxide synthase inhibition on cerebral arterioles in Wistar-Kyoto rats

Journal article

Peer reviewed

Effects of chronic nitric oxide synthase inhibition on cerebral arterioles in Wistar-Kyoto rats

Jean-Marc Chillon and Gary L Baumbach

Journal of hypertension, Vol.22(3), pp.529-534

03/2004

DOI: 10.1097/00004872-200403000-00015

PMID: 15076158

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Abstract

Cerebral arterioles in stroke-prone spontaneously hypertensive rats (SHRSP), but not in Sprague-Dawley rats with hypertension induced by nitric oxide (NO) synthase inhibition, undergo inward remodeling. The goal of this study was to determine whether development of vascular inward remodeling may depend on genetic factors. We examined effects of NO synthase inhibition on the structure of cerebral arterioles in Wistar-Kyoto rats (WKY), a rat strain genetically distinct from Sprague-Dawley. Pressure (servonull), diameter (cranial window) and cross-sectional area of the vessel wall (CSA, histologically) were measured in maximally dilated (EDTA) cerebral arterioles in WKY, untreated (n = 8) or treated for 3 months with the NO synthase inhibitor N-nitro-L-arginine methyl ester (L-NAME) (10 mg/kg per day, n = 10) in the drinking water, and in untreated SHRSP (n = 7). Treatment with L-NAME in WKY increased mean cerebral arteriolar pressure (69 +/- 7 versus 47 +/- 7 mmHg, P < 0.05) and pulse pressure (30 +/- 3 versus 17 +/- 1 mmHg, P < 0.05) to levels significantly lower than in SHRSP (98 +/- 5 and 35 +/- 1 mmHg respectively, P < 0.05). CSA was significantly greater in L-NAME-treated WKY and SHRSP than in untreated WKY (1692 +/- 50 and 1525 +/- 98 microm respectively, versus 1224 +/- 85, P < 0.05). External diameter was significantly less in L-NAME-treated WKY than in untreated WKY (119 +/- 5 versus 135 +/- 4 microm, P < 0.05) but significantly greater than in SHRSP (98 +/- 1 microm, P < 0.05). Cerebral arterioles undergo hypertrophy and remodeling in WKY with L-NAME-induced hypertension. These findings suggest that genetic factors present in WKY and SHRSP may play a role in the development of vascular inward remodeling during chronic hypertension in rats.

Animals

Arterioles - drug effects

Arterioles - physiology

Blood Pressure - drug effects

Body Weight

Cerebral Arteries - drug effects

Cerebral Arteries - physiology

Cerebrovascular Circulation - drug effects

Enzyme Inhibitors - pharmacology

Hypertension - metabolism

Hypertension - physiopathology

Male

NG-Nitroarginine Methyl Ester - pharmacology

Nitric Oxide Synthase - antagonists & inhibitors

Rats

Rats, Inbred SHR

Rats, Inbred WKY

Details

Title: Subtitle: Effects of chronic nitric oxide synthase inhibition on cerebral arterioles in Wistar-Kyoto rats
Creators: Jean-Marc Chillon - Cardiovascular Research Group, Faculté de Pharmacie de l'Université Henri Poincaré-Nancy I, Nancy, France. chillon@pharma.uhp-nancy.fr
Gary L Baumbach
Resource Type: Journal article
Publication Details: Journal of hypertension, Vol.22(3), pp.529-534
DOI: 10.1097/00004872-200403000-00015
PMID: 15076158
ISSN: 0263-6352
eISSN: 1473-5598
Grant note: HL-16066 / NHLBI NIH HHS HL-22149 / NHLBI NIH HHS NS-24621 / NINDS NIH HHS HL-14388 / NHLBI NIH HHS
Language: English
Date published: 03/2004
Academic Unit: Pathology; Fraternal Order of Eagles Diabetes Research Center
Record Identifier: 9984186617602771

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