Journal article
Effects of clinical and environmental factors on bronchoalveolar antibody responses to Pneumocystis jirovecii: A prospective cohort study of HIV+ patients
PloS one, Vol.12(7), pp.e0180212-e0180212
2017
DOI: 10.1371/journal.pone.0180212
PMCID: PMC5503245
PMID: 28692651
Abstract
Humoral immunity plays an important role against Pneumocystis jirovecii infection, yet clinical and environmental factors that impact bronchoalveolar antibody responses to P. jirovecii remain uncertain.
From October 2008-December 2011 we enrolled consecutive HIV-infected adults admitted to San Francisco General Hospital (SFGH) who underwent bronchoscopy for suspected Pneumocystis pneumonia (PCP). We used local air quality monitoring data to assign ozone, nitrogen dioxide, and fine particulate matter exposures within 14 days prior to hospital admission. We quantified serum and bronchoalveolar lavage fluid (BALF) antibody responses to P. jirovecii major surface glycoprotein (Msg) recombinant constructs using ELISA. We then fit linear regression models to determine whether PCP and ambient air pollutants were associated with bronchoalveolar antibody responses to Msg.
Of 81 HIV-infected patients enrolled, 47 (58%) were diagnosed with current PCP and 9 (11%) had a prior history of PCP. The median CD4+ count was 51 cells/μl (IQR 15-129) and 44% were current smokers. Serum antibody responses to Msg were statistically significantly predictive of BALF antibody responses, with the exception of IgG responses to MsgC8 and MsgC9. Prior PCP was associated with increased BALF IgA responses to Msg and current PCP was associated with decreased IgA responses. For instance, among patients without current PCP, those with prior PCP had a median 73.2 U (IQR 19.2-169) IgA response to MsgC1 compared to a 5.00 U (3.52-12.6) response among those without prior PCP. Additionally, current PCP predicted a 22.5 U (95%CI -39.2, -5.82) lower IgA response to MsgC1. Ambient ozone within the two weeks prior to hospital admission was associated with decreased BALF IgA responses to Msg while nitrogen dioxide was associated with increased IgA responses.
PCP and ambient air pollutants were associated with BALF IgA responses to P. jirovecii in HIV-infected patients evaluated for suspected PCP.
Details
- Title: Subtitle
- Effects of clinical and environmental factors on bronchoalveolar antibody responses to Pneumocystis jirovecii: A prospective cohort study of HIV+ patients
- Creators
- Robert J Blount - Division of Pediatric Pulmonology, University of California, San Francisco, California, United States of AmericaKieran R Daly - Veterans Administration Medical Center, Cincinnati, Ohio, United States of AmericaSerena Fong - HIV/AIDS Division, San Francisco General Hospital, University of California, San Francisco, California, United States of AmericaEmily Chang - HIV/AIDS Division, San Francisco General Hospital, University of California, San Francisco, California, United States of AmericaKatherine Grieco - HIV/AIDS Division, San Francisco General Hospital, University of California, San Francisco, California, United States of AmericaMeredith Greene - HIV/AIDS Division, San Francisco General Hospital, University of California, San Francisco, California, United States of AmericaStephen Stone - HIV/AIDS Division, San Francisco General Hospital, University of California, San Francisco, California, United States of AmericaJohn Balmes - Environmental Health Sciences, University of California, Berkeley, California, United States of AmericaRobert F Miller - Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United KingdomPeter D Walzer - Veterans Administration Medical Center, Cincinnati, Ohio, United States of AmericaLaurence Huang - HIV/AIDS Division, San Francisco General Hospital, University of California, San Francisco, California, United States of America
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.12(7), pp.e0180212-e0180212
- DOI
- 10.1371/journal.pone.0180212
- PMID
- 28692651
- PMCID
- PMC5503245
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library of Science
- Grant note
- R01 HL128156 / NHLBI NIH HHS K23 ES025807 / NIEHS NIH HHS T32 HL007185 / NHLBI NIH HHS R01 HL090335 / NHLBI NIH HHS F32 ES022582 / NIEHS NIH HHS P30 AI027763 / NIAID NIH HHS K24 HL087713 / NHLBI NIH HHS
- Language
- English
- Date published
- 2017
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Occupational and Environmental Health; Stead Family Department of Pediatrics; Internal Medicine
- Record Identifier
- 9984093224902771
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