Journal article
Effects of colonization, luminescence, and autoinducer on host transcription during development of the squid-vibrio association
Proceedings of the National Academy of Sciences - PNAS, Vol.105(32), pp.11323-11328
08/12/2008
DOI: 10.1073/pnas.0802369105
PMCID: PMC2516268
PMID: 18682555
Abstract
The light-organ symbiosis between the squid Euprymna scolopes and the luminous bacterium Vibrio fischeri offers the opportunity to decipher the hour-by-hour events that occur during the natural colonization of an animal's epithelial surface by its microbial partners. To determine the genetic basis of these events, a glass-slide microarray was used to characterize the light-organ transcriptome of juvenile squid in response to the initiation of symbiosis. Patterns of gene expression were compared between animals not exposed to the symbiont, exposed to the wild-type symbiont, or exposed to a mutant symbiont defective in either of two key characters of this association: bacterial luminescence or autoinducer (AI) production. Hundreds of genes were differentially regulated as a result of symbiosis initiation, and a hierarchy existed in the magnitude of the host's response to three symbiont features: bacterial presence > luminescence > AI production. Putative host receptors for bacterial surface molecules known to induce squid development are up-regulated by symbiont light production, suggesting that bioluminescence plays a key role in preparing the host for bacteria-induced development. Further, because the transcriptional response of tissues exposed to AI in the natural context (i.e., with the symbionts) differed from that to AI alone, the presence of the bacteria potentiates the role of quorum signals in symbiosis. Comparison of these microarray data with those from other symbioses, such as germ-free/conventionalized mice and zebrafish, revealed a set of shared genes that may represent a core set of ancient host responses conserved throughout animal evolution.
Details
- Title: Subtitle
- Effects of colonization, luminescence, and autoinducer on host transcription during development of the squid-vibrio association
- Creators
- Carlene K Chun - Department of Medical Microbiology and Immunology, University of Wisconsin, Madison, WI 53706, USAJoshua V TrollIrina KorolevaBartley BrownLiliana ManzellaEinat SnirHakeem AlmabraziTodd E ScheetzMaria de Fatima BonaldoThomas L CasavantM Bento SoaresEdward G RubyMargaret J McFall-Ngai
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.105(32), pp.11323-11328
- Publisher
- United States
- DOI
- 10.1073/pnas.0802369105
- PMID
- 18682555
- PMCID
- PMC2516268
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Grant note
- R01 AI050661 / NIAID NIH HHS R01 RR012294 / NCRR NIH HHS R01-AI50661 / NIAID NIH HHS R01-RR12294 / NCRR NIH HHS
- Language
- English
- Date published
- 08/12/2008
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Ophthalmology and Visual Sciences
- Record Identifier
- 9983979982702771
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