Journal article
Effects of estradiol, PCB3, and their hydroxylated metabolites on proliferation, cell cycle, and apoptosis of human breast cancer cells
Environmental toxicology and pharmacology, Vol.25(2), pp.227-233
2008
DOI: 10.1016/j.etap.2007.10.004
PMID: 21783862
Abstract
Certain estradiol metabolites and industrial pollutants, like polychlorinated biphenyls, may play a more important role in enhancing breast cancer risk than 17β-estradiol. The aim of this study was to compare the effects of 17β-estradiol (E2) with that of the air pollutant 4-chlorobiphenyl (PCB3) and four of their hydroxylated metabolites on cell cycle, proliferation, and apoptosis in MCF-7 human breast cancer cells at concentrations of 0.1–10
nM (E2, 2-OH-E2, and 4-OH-E2) and 0.3–300
nM (PCB3, 4-OH-PCB3, and 3, 4-diOH-PCB3) and 24–260
h of exposure. E2 increased cell proliferation and cells in S-phase at all time points. 2-OH-E2 and 4-OH-E2 had no effect on the cell cycle, but a stimulatory action on cell proliferation from 72 to 260
h of exposure to 4-OH-E2 and at 260
h to 2-OH-E2 was seen. E2 and its metabolites had no effect on apoptosis. PCB3 and 4-OH-PCB3 showed no effect on proliferation, apoptosis or cell cycle distribution at any concentration and time point. Longer time exposures to 3,4-di-OH-PCB at 300
nM caused a decrease of cells and an increase in G2/M and apoptotic cells. These results confirm the proliferative effect of E2 and its metabolite 4-OH-E2 in estrogen receptor positive breast cancer cells, but show no mitogenic activity for PCB3 and 4-OH-PCB3. However, the cell cycle and apoptosis effects of 3,4-diOH-PCB3 need further analysis.
Details
- Title: Subtitle
- Effects of estradiol, PCB3, and their hydroxylated metabolites on proliferation, cell cycle, and apoptosis of human breast cancer cells
- Creators
- Ewa L Gregoraszczuk - Department of Physiology and Toxicology of Reproduction, Chair of Animal Physiology, Institute of Zoology, Jagiellonian University, Kraków, PolandA Rak - Department of Physiology and Toxicology of Reproduction, Chair of Animal Physiology, Institute of Zoology, Jagiellonian University, Kraków, PolandGabriele Ludewig - The University of Iowa, Department of Occupational and Environmental Health, College of Public Health, 100 Oakdale Campus 124 IREH, Iowa City, IA, USAAnna Gasińska - Department of Applied Radiobiology, Center of Oncology, Maria Sklodowska-Curie Memorial Institute, Krakow, Poland
- Resource Type
- Journal article
- Publication Details
- Environmental toxicology and pharmacology, Vol.25(2), pp.227-233
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.etap.2007.10.004
- PMID
- 21783862
- ISSN
- 1382-6689
- eISSN
- 1872-7077
- Language
- English
- Date published
- 2008
- Academic Unit
- Occupational and Environmental Health; Iowa Superfund Research Program
- Record Identifier
- 9983997481102771
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