Effects of fludrocortisone on water and sodium intake of C57BL/6 mice

Ralph F Johnson; Terry G Beltz; Alan Kim Johnson; Robert L Thunhorst

doi:10.1152/ajpregu.00033.2015

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Effects of fludrocortisone on water and sodium intake of C57BL/6 mice

Journal article

Open access

Peer reviewed

Effects of fludrocortisone on water and sodium intake of C57BL/6 mice

Ralph F Johnson, Terry G Beltz, Alan Kim Johnson and Robert L Thunhorst

American journal of physiology. Regulatory, integrative and comparative physiology, Vol.309(3), pp.R247-R254

08/01/2015

DOI: 10.1152/ajpregu.00033.2015

PMCID: PMC4525329

PMID: 26017491

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Abstract

Little is known about steroidal control of thirst- and salt-appetite behaviors of mice. The current study investigates effects of fludrocortisone acetate (FCA), a steroid with potent glucocorticoid and mineralocorticoid effects, on thirst- and salt-appetite responses of C57BL/6 mice. Treatment with FCA produced dose-dependent (5, 10, and 25 mg/kg) increases in both magnitude and duration of water and sodium intake. Chronic elevation of water and saline intake was achieved with daily injections of FCA. Daily injection of FCA, when only 0.9% saline was available, produced a remarkably rapid increase in saline intake. A single injection of FCA stimulated brisk diuresis and natriuresis in fluid-restricted animals. This work is the first to demonstrate copious water drinking by mice in response to FCA. The results are discussed in terms of the possibility that the renal effects of FCA promote increases in water and sodium turnover and thereby, increases in water and sodium ingestion.

Water-Electrolyte Balance - physiology

Sodium Chloride - pharmacology

Mice, Inbred C57BL

Water - metabolism

Male

Water-Electrolyte Balance - drug effects

Fludrocortisone - analogs & derivatives

Fludrocortisone - pharmacology

Thirst - physiology

Thirst - drug effects

Animals

Drinking Behavior - drug effects

Appetite - physiology

Drinking Behavior - physiology

Sodium, Dietary - metabolism

Details

Title: Subtitle: Effects of fludrocortisone on water and sodium intake of C57BL/6 mice
Creators: Ralph F Johnson - Department of Psychological and Brain Sciences, University of Iowa, Iowa City, Iowa
Terry G Beltz - Department of Psychological and Brain Sciences, University of Iowa, Iowa City, Iowa
Alan Kim Johnson - Department of Psychological and Brain Sciences, University of Iowa, Iowa City, Iowa; Department of Health and Human Physiology, University of Iowa, Iowa City, Iowa; Department of Pharmacology, University of Iowa, Iowa City, Iowa; and François M. Abboud Cardiovascular Center, University of Iowa, Iowa City, Iowa
Robert L Thunhorst - Department of Psychological and Brain Sciences, University of Iowa, Iowa City, Iowa; François M. Abboud Cardiovascular Center, University of Iowa, Iowa City, Iowa robert-thunhorst@uiowa.edu
Resource Type: Journal article
Publication Details: American journal of physiology. Regulatory, integrative and comparative physiology, Vol.309(3), pp.R247-R254
DOI: 10.1152/ajpregu.00033.2015
PMID: 26017491
PMCID: PMC4525329
NLM abbreviation: Am J Physiol Regul Integr Comp Physiol
ISSN: 0363-6119
eISSN: 1522-1490
Publisher: United States
Grant note: MH59239 / NIMH NIH HHS HL098207 / NHLBI NIH HHS AG25465 / NIA NIH HHS P01 HL014388 / NHLBI NIH HHS HL14388 / NHLBI NIH HHS MH80241 / NIMH NIH HHS R01 AG025465 / NIA NIH HHS
Language: English
Date published: 08/01/2015
Academic Unit: Psychological and Brain Sciences; Neuroscience and Pharmacology; Health, Sport, and Human Physiology
Record Identifier: 9984002335802771

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