Effects of fluoride-modified titanium surfaces on osteoblast proliferation and gene expression

Zakiah M Isa; Galen B Schneider; Rebecca Zaharias; Denise Seabold; Clark M Stanford

Back

Effects of fluoride-modified titanium surfaces on osteoblast proliferation and gene expression

Journal article

Peer reviewed

Effects of fluoride-modified titanium surfaces on osteoblast proliferation and gene expression

Zakiah M Isa, Galen B Schneider, Rebecca Zaharias, Denise Seabold and Clark M Stanford

The International journal of oral and maxillofacial implants, Vol.21(2), pp.203-211

03/2006

PMID: 16634490

View Online

Abstract

The objective of this study was to test the hypothesis that fluoride-modified titanium surfaces would enhance osteoblast differentiation. Osteoblast growth on a moderately rough etched fluoride-modified titanium surface (alteration in cellular differentiation) was compared to osteoblast growth on the same surface grit-blasted with titanium dioxide. The potential role of nanometer-level alterations on cell shape and subsequent differentiation was then compared. Human embryonic palatal mesenchymal (HEPM) cultures were incubated on the respective surfaces for 1, 3, and 7 days, followed by analysis for cell proliferation, alkaline phosphatase (ALP) -specific activity, and mRNA steady-state expression for bone-related genes (ALP, type I collagen, osteocalcin, bone sialoprotein [BSP] II, Cbfa1, and osterix) by real-time polymerase chain reaction (PCR). The different surfaces did not alter the mRNA expression for ALP, type I collagen, osterix, osteocalcin, or BSP II. However, Cbfa1 expression on the fluoride-modified titanium surface was significantly higher (P < .001) at 1 week. The number of cells on this surface was 20% lower than the number of cells on the surface TiO2-blasted with 25-microm particles but not significantly different from the number of cells on the surface TiO2-blasted with 125-microm particles. Cells grown on all the titanium surfaces expressed similar levels of ALP activity. The results indicated that a fluoride-modified surface topography, in synergy with surface roughness, may have a greater influence on the level of expression of Cbfa1 (a key regulator for osteogenesis) than the unmodified titanium surfaces studied.

Mesenchymal Stromal Cells - drug effects

Microscopy, Electron, Scanning

Gene Expression - drug effects

Humans

Osteoblasts - drug effects

Cells, Cultured

Coated Materials, Biocompatible - pharmacology

Titanium - chemistry

Cell Adhesion - drug effects

Fluorides - pharmacology

Analysis of Variance

Cell Differentiation - drug effects

Core Binding Factor Alpha 1 Subunit - biosynthesis

Surface Properties

Cell Proliferation - drug effects

Acid Etching, Dental

Osteoblasts - metabolism

Details

Title: Subtitle: Effects of fluoride-modified titanium surfaces on osteoblast proliferation and gene expression
Creators: Zakiah M Isa - Department of Prosthetic Dentistry, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia
Galen B Schneider
Rebecca Zaharias
Denise Seabold
Clark M Stanford
Resource Type: Journal article
Publication Details: The International journal of oral and maxillofacial implants, Vol.21(2), pp.203-211
PMID: 16634490
NLM abbreviation: Int J Oral Maxillofac Implants
ISSN: 0882-2786
eISSN: 1942-4434
Publisher: United States
Grant note: R03DE014269 / NIDCR NIH HHS
Language: English
Date published: 03/2006
Academic Unit: Anatomy and Cell Biology; Dentistry Administration; Prosthodontics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center
Record Identifier: 9984066084902771

Metrics

21 Record Views

93 Times Cited - Web of Science