Journal article
Effects of gap junction blockers on human neocortical synchronization
Neurobiology of disease, Vol.22(3), pp.496-508
2006
DOI: 10.1016/j.nbd.2005.12.011
PMID: 16478664
Abstract
Field potentials and intracellular recordings were obtained from human neocortical slices to study the role of gap junctions (GJ) in neuronal network synchronization. First, we examined the effects of GJ blockers (i.e., carbenoxolone, octanol, quinine, and quinidine) on the spontaneous synchronous events (duration = 0.2–1.1 s; intervals of occurrence = 3–27 s) generated by neocortical slices obtained from temporal lobe epileptic patients during application of 4-aminopyridine (4AP, 50 μM) and glutamatergic receptor antagonists. The synchronicity of these potentials (recorded at distances up to 5 mm) was decreased by GJ blockers within 20 min of application, while prolonged GJ blockers treatment at higher doses made them disappear with different time courses. Second, we found that slices from patients with focal cortical dysplasia (FCD) could generate in normal medium spontaneous synchronous discharges (duration = 0.4–8 s; intervals of occurrence = 0.5–90 s) that were (i) abolished by NMDA receptor antagonists and (ii) slowed down by carbenoxolone. Finally, octanol or carbenoxolone blocked 4AP-induced ictal-like discharges (duration = up to 35 s) in FCD slices. These data indicate that GJ play a role in synchronizing human neocortical networks and may implement epileptiform activity in FCD.
Details
- Title: Subtitle
- Effects of gap junction blockers on human neocortical synchronization
- Creators
- S Gigout - INSERM U 573, Paris, 75014 FranceJ Louvel - INSERM U 573, Paris, 75014 FranceH Kawasaki - Montreal Neurological Institute and Departments of Neurology and Neurosurgery and of Physiology, McGill University, Montreal, Quebec, Canada H3A 2B4M D'Antuono - Montreal Neurological Institute and Departments of Neurology and Neurosurgery and of Physiology, McGill University, Montreal, Quebec, Canada H3A 2B4V Armand - INSERM U 573, Paris, 75014 FranceI Kurcewicz - INSERM U 573, Paris, 75014 FranceA Olivier - Montreal Neurological Institute and Departments of Neurology and Neurosurgery and of Physiology, McGill University, Montreal, Quebec, Canada H3A 2B4J Laschet - INSERM U 573, Paris, 75014 FranceB Turak - Hôpital Sainte-Anne, Paris, 75014 FranceB Devaux - Hôpital Sainte-Anne, Paris, 75014 FranceR Pumain - INSERM U 573, Paris, 75014 FranceM Avoli - Montreal Neurological Institute and Departments of Neurology and Neurosurgery and of Physiology, McGill University, Montreal, Quebec, Canada H3A 2B4
- Resource Type
- Journal article
- Publication Details
- Neurobiology of disease, Vol.22(3), pp.496-508
- DOI
- 10.1016/j.nbd.2005.12.011
- PMID
- 16478664
- NLM abbreviation
- Neurobiol Dis
- ISSN
- 0969-9961
- eISSN
- 1095-953X
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2006
- Academic Unit
- Neurosurgery
- Record Identifier
- 9984083276902771
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