Journal article
Effects of implant microtopography on osteoblast cell attachment
Implant dentistry, Vol.12(2), pp.175-181
2003
DOI: 10.1097/01.ID.0000058309.77613.87
PMID: 12861887
Abstract
The overall aim of this project was to study osteoblast cell attachment on titanium surfaces with varying surface roughness.
Commercially pure titanium surfaces were prepared by polishing through 600-grit sandpaper, sandblasting, or sandblasting followed by acid etching to produce surfaces of varying roughness, as determined by scanning electron microscopy and atomic force microscopy. In vitro cell attachment of MC3T3-E1 osteoblasts was performed on the prepared surfaces in both serum-containing and serum-free media conditions.
Cell attachment was directly related to the average surface roughness, with the highest levels of cell attachment observed on sandblasted and sandblasted-acidetched surfaces. Similar patterns of cell attachment were observed when serum-free conditions were employed.
Combined surface analytical and cell/molecular biological techniques are powerful tools to broaden our understanding of biological events occurring at the implant-tissue interface. Data acquired from these in vitro techniques provide a translational application to in vivo clinical models leading to the next generation of dental implants.
Details
- Title: Subtitle
- Effects of implant microtopography on osteoblast cell attachment
- Creators
- John C Keller - Dows Institute for Dental Research, College of Dentistry, N407 Dental Science Building, University of Iowa, Iowa City, IA 52242-1010, USA. John-Keller@uiowa.eduGalen B SchneiderClark M StanfordBradley Kellogg
- Resource Type
- Journal article
- Publication Details
- Implant dentistry, Vol.12(2), pp.175-181
- DOI
- 10.1097/01.ID.0000058309.77613.87
- PMID
- 12861887
- NLM abbreviation
- Implant Dent
- ISSN
- 1056-6163
- eISSN
- 1538-2982
- Publisher
- United States
- Grant note
- P30 DE10126 / NIDCR NIH HHS T32 DE07159 / NIDCR NIH HHS P60 DE13076 / NIDCR NIH HHS
- Language
- English
- Date published
- 2003
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Graduate College Admin and Gen; President; Dentistry Administration; Prosthodontics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Dental Research
- Record Identifier
- 9984065722402771
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