Effects of implant surface microtopography on osteoblast gene expression

Chihiro Masaki; Galen B Schneider; Rebecca Zaharias; Denise Seabold; Clark Stanford

doi:10.1111/j.1600-0501.2005.01170.x

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Effects of implant surface microtopography on osteoblast gene expression

Journal article

Peer reviewed

Effects of implant surface microtopography on osteoblast gene expression

Chihiro Masaki, Galen B Schneider, Rebecca Zaharias, Denise Seabold and Clark Stanford

Clinical oral implants research, Vol.16(6), pp.650-656

12/2005

DOI: 10.1111/j.1600-0501.2005.01170.x

PMID: 16307571

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Abstract

The promotion of osteoblast attachment and differentiation has been evaluated on various implant surfaces. However, the effects of different implant surface properties on gene expression of key osteogenic factors are not fully understood. The objectives of this study were to evaluate how topographical effects on titanium surface alter the expression of bone-related genes and transcription factors. Osteoblasts were cultured on titanium disks prepared with a titanium dioxide grit blasting (TiOBlast) or grit blasted and etched with hydrofluoric acid (Osseospeed), grit blasted and etched (SLA-1), or grit blasted, etched and rinsed with N2 protection and stored in isotonic NaCl (SLA-2) commercially pure titanium implant discs. High-density cultures of human mesenchymal pre-osteoblastic cells (HEPM 1486, ATCC) were grown for 72 h and real-time PCR used for quantitative analysis of alkaline phosphatase (ALP), core-binding factor alpha1 (Cbfa1), Osterix, Type I Collagen, Osteocalcin and bone sialoprotein II gene expression. Real-time PCR showed significant (P<0.001) increases in ALP gene expression in osteoblasts grown on SLA-2, relative to all other surfaces. Cbfa1/RUNX-2 gene expression was significantly (P<0.01) increased on Osseospeed and TiOBlast surface as compared with SLA-1 and SLA-2 surfaces. The expression of Osterix had a trend similar to that of Cbfa1. In conclusion, implant surface properties may contribute to the regulation of osteoblast differentiation by influencing the level of bone-related genes and transcription factors in human mesenchymal pre-osteoblastic cells.

Gene Expression

Osteocalcin - biosynthesis

Osseointegration - genetics

Integrin-Binding Sialoprotein

Humans

Cells, Cultured

Transcription Factors - biosynthesis

Coated Materials, Biocompatible

Cell Adhesion

Dental Etching

Sialoglycoproteins - biosynthesis

Collagen Type I - biosynthesis

Core Binding Factor Alpha 1 Subunit - biosynthesis

Surface Properties

Sp7 Transcription Factor

Titanium

Cell Differentiation

Osteoblasts - cytology

Osteoblasts - metabolism

Details

Title: Subtitle: Effects of implant surface microtopography on osteoblast gene expression
Creators: Chihiro Masaki - Department of Advanced Prosthodontics, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
Galen B Schneider
Rebecca Zaharias
Denise Seabold
Clark Stanford
Resource Type: Journal article
Publication Details: Clinical oral implants research, Vol.16(6), pp.650-656
DOI: 10.1111/j.1600-0501.2005.01170.x
PMID: 16307571
NLM abbreviation: Clin Oral Implants Res
ISSN: 0905-7161
eISSN: 1600-0501
Publisher: Denmark
Grant note: DE13076 / NIDCR NIH HHS DE14269 / NIDCR NIH HHS
Language: English
Date published: 12/2005
Academic Unit: Dentistry Administration; Prosthodontics; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center
Record Identifier: 9984065707002771

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