Journal article
Effects of lithium chloride on the gene expression profiles in Drosophila heads
Neuroscience research, Vol.64(4), pp.413-420
2009
DOI: 10.1016/j.neures.2009.04.015
PMCID: PMC2743107
PMID: 19410610
Abstract
To gain insight into the basic neurobiological processes regulated by lithium—an effective drug for bipolar disorder—we used Affymetrix Genome Arrays to examine lithium-induced changes in genome-wide gene expression profiles of head mRNA from the genetic model organism
Drosophila melanogaster. First, to identify the individual genes whose transcript levels are most significantly altered by lithium, we analyzed the microarray data with stringent criteria (fold change
>
2;
p
<
0.001) and evaluated the results by RT-PCR. This analysis identified 12 genes that encode proteins with various biological functions, including an enzyme responsible for amino acid metabolism and a putative amino acid transporter. Second, to uncover the biological pathways involved in lithium's action in the nervous system, we used less stringent criteria (fold change
>
1.2; FDR
<
0.05) and assigned the identified 66 lithium-responsive genes to biological pathways using DAVID (Database for Annotation, Visualization and Integrated Discovery). The gene ontology categories most significantly affected by lithium were amino acid metabolic processes. Taken together, these data suggest that amino acid metabolism is important for lithium's actions in the nervous system, and lay a foundation for future functional studies of lithium-responsive neurobiological processes using the versatile molecular and genetic tools that are available in
Drosophila.
Details
- Title: Subtitle
- Effects of lithium chloride on the gene expression profiles in Drosophila heads
- Creators
- Junko Kasuya - Department of Anesthesia, College of Medicine, University of Iowa, Iowa City, IA 52242, United StatesGarrett Kaas - Interdisciplinary Graduate Program in Genetics, University of Iowa, United StatesToshihiro Kitamoto - Department of Anesthesia, College of Medicine, University of Iowa, Iowa City, IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Neuroscience research, Vol.64(4), pp.413-420
- DOI
- 10.1016/j.neures.2009.04.015
- PMID
- 19410610
- PMCID
- PMC2743107
- NLM abbreviation
- Neurosci Res
- ISSN
- 0168-0102
- eISSN
- 1872-8111
- Publisher
- Elsevier Ireland Ltd
- Language
- English
- Date published
- 2009
- Academic Unit
- Iowa Neuroscience Institute; Anesthesia; Neuroscience and Pharmacology
- Record Identifier
- 9984007294202771
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