Journal article
Effects of long-term ethanol ingestion on hepatic iron metabolism in two mouse strains
Clinical and experimental pharmacology & physiology, Vol.48(4), pp.534-542
04/01/2021
DOI: 10.1111/1440-1681.13445
PMID: 33319364
Abstract
The mechanisms responsible for dysregulation of iron metabolism in response to ethanol ingestion are poorly understood. Relatively brief ethanol exposures in rodents are associated with reduced hepatic hepcidin expression without increases in hepatic iron content. This study evaluated the effects of long-term ethanol treatment on hepatic iron metabolism in two mouse strains. Ethanol was administered in the drinking water to C57BL/6 and BALB/c mice for up to 11 months. Hepatic histology and iron concentrations (HIC) were assessed, along with expression of relevant genes and proteins by real-time RT-PCR and western blot, respectively. The livers of ethanol-consuming mice of both strains showed mild steatosis without inflammation or fibrosis. Stainable hepatocyte iron was modestly increased in both strains ingesting ethanol, although hepatic iron concentrations were significantly higher only in C57BL/6 mice. Long-term ethanol did not affect hepcidin mRNA (Hamp1 or Hamp2) in either strain, nor was the expression of several oxidative stress-responsive genes (glutamate cysteine ligase, gamma-glutamyl transpeptidase, heme oxygenase-1 and growth differentiation factor 15) altered in response to ethanol, suggesting that oxidative stress and suppression of hepcidin expression in short-term ethanol feeding models may be transient phenomena that resolve as mice adapt to ethanol exposure. This murine model of chronic ethanol ingestion demonstrates modest increases in hepatic iron without changes in hepcidin expression, markers of oxidative stress or significant histologic liver injury. Further investigations are needed to characterize the mechanisms of dysregulated iron metabolism resulting from chronic ethanol ingestion.
Details
- Title: Subtitle
- Effects of long-term ethanol ingestion on hepatic iron metabolism in two mouse strains
- Creators
- Steven A. Bloomer - Penn State AbingtonKimberly A. Broadhurst - University of IowaM. Maleah Mathahs - Univ Iowa, Dept Internal Med, Carver Coll Med, Div Gastroenterol Hepatol, Iowa City, IA 52242 USAKyle E. Brown - United States Department of Veterans Affairs
- Resource Type
- Journal article
- Publication Details
- Clinical and experimental pharmacology & physiology, Vol.48(4), pp.534-542
- DOI
- 10.1111/1440-1681.13445
- PMID
- 33319364
- NLM abbreviation
- Clin Exp Pharmacol Physiol
- ISSN
- 0305-1870
- eISSN
- 1440-1681
- Publisher
- Wiley
- Number of pages
- 9
- Grant note
- VA Merit Review grant; US Department of Veterans Affairs AA014405 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 04/01/2021
- Academic Unit
- Gastroenterology and Hepatology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984312981502771
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