Effects of microcin 24-producing Escherichia coli on shedding and multiple-antimicrobial resistance of Salmonella enterica serotype typhimurium in pigs

Timothy S Frana; Steven A Carlson; Daryl C Rauser; Bradley D Jones; Brian J Fergen; Ronald W Griffith

doi:10.2460/ajvr.2004.65.1616

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Effects of microcin 24-producing Escherichia coli on shedding and multiple-antimicrobial resistance of Salmonella enterica serotype typhimurium in pigs

Journal article

Peer reviewed

Effects of microcin 24-producing Escherichia coli on shedding and multiple-antimicrobial resistance of Salmonella enterica serotype typhimurium in pigs

Timothy S Frana, Steven A Carlson, Daryl C Rauser, Bradley D Jones, Brian J Fergen and Ronald W Griffith

American journal of veterinary research, Vol.65(12), pp.1616-1620

12/2004

DOI: 10.2460/ajvr.2004.65.1616

PMID: 15631023

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Abstract

To investigate the effect of an Escherichia that produced microcin 24 (Mcc24) on shedding of of Salmonella enterica serotypeTyphimurium in swine and evaluate evidence of in vivo activation of the Mcc24-mediated, multiple-antibiotic resistance (mar) operon. 36 crossbred weaned pigs. 24 pigs were allocated to 2 groups (12 pigs/group). Pigs in 1 group received daily oral administration of an Mcc24-producing E coli, whereas the other group received a non-Mcc24-producing E coli. All pigs were challenge exposed with Salmonella Typhimurium chi4232. A third group of 6 pigs received Mcc24-producing E coli and was challenge exposed with an Mcc24-sensitive, marA-deleted strain of Salmonella Typhimurium 4232. After challenge exposure, fecal samples from all pigs were cultured to detect shedding of Salmonella Typhimurium and Salmonella Typhimurium isolates were screened for resistance to ciprofloxacin. Fecal samples were collected throughout the study, and tissue samples were collected during necropsy. Differences in shedding of Salmonella Typhimurium were not detected between groups receiving Mcc24-producing or non-Mcc24-producing E coli. No significant differences were found in quantitative analysis between groups receiving Mcc24-producing and non-Mcc24-producing E coli. Evidence of mar activation was not detected. Microcin-producing E coli did not exert an effect on shedding of SalmonellaTyphimurium or mar activation in pigs. It may be difficult or impractical to create the conditions required for Mcc24 to be an effective part of a food safety intervention to reduce shedding of Salmonella Typhimurium.

Peptides

Drug Resistance, Multiple

Ciprofloxacin - pharmacology

Male

Colony Count, Microbial

RNA, Messenger - metabolism

Feces - microbiology

Bacteriocins - genetics

Salmonella typhimurium - isolation & purification

Animals

Polymerase Chain Reaction - veterinary

Swine

Cloning, Molecular

Drug Resistance, Bacterial - physiology

Salmonella Infections, Animal - microbiology

Female

Swine Diseases - microbiology

Anti-Bacterial Agents - biosynthesis

Anti-Bacterial Agents - pharmacology

Salmonella typhimurium - drug effects

Bacteriocins - biosynthesis

Escherichia coli - physiology

Details

Title: Subtitle: Effects of microcin 24-producing Escherichia coli on shedding and multiple-antimicrobial resistance of Salmonella enterica serotype typhimurium in pigs
Creators: Timothy S Frana - Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50010, USA
Steven A Carlson
Daryl C Rauser
Bradley D Jones
Brian J Fergen
Ronald W Griffith
Resource Type: Journal article
Publication Details: American journal of veterinary research, Vol.65(12), pp.1616-1620
DOI: 10.2460/ajvr.2004.65.1616
PMID: 15631023
NLM abbreviation: Am J Vet Res
ISSN: 0002-9645
eISSN: 1943-5681
Language: English
Date published: 12/2004
Academic Unit: Microbiology and Immunology
Record Identifier: 9984083831102771

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