Effects of nicotine on pulmonary surfactant proteins A and D in ovine lung epithelia

Tatjana Lazic; Milan Matic; Jack M Gallup; Albert Van Geelen; David K Meyerholz; Branka Grubor; Paula M Imerman; Marcia M.M.A de-Macedo; Mark R Ackermann

doi:10.1002/ppul.21153

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Effects of nicotine on pulmonary surfactant proteins A and D in ovine lung epithelia

Journal article

Peer reviewed

Effects of nicotine on pulmonary surfactant proteins A and D in ovine lung epithelia

Tatjana Lazic, Milan Matic, Jack M Gallup, Albert Van Geelen, David K Meyerholz, Branka Grubor, Paula M Imerman, Marcia M.M.A de-Macedo and Mark R Ackermann

Pediatric pulmonology, Vol.45(3), pp.255-262

03/2010

DOI: 10.1002/ppul.21153

PMCID: PMC2981073

PMID: 20131324

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Abstract

Maternal smoking during pregnancy increases the incidence and severity of respiratory infections in neonates. Surfactant proteins A and D (SP-A and SP-D, respectively) are components of pulmonary innate immunity and have an important role in defense against inhaled pathogens. The purpose of this study was to determine if nicotine exposure during the third trimester of pregnancy alters the expression of SP-A and SP-D of fetal lung epithelia. Pregnant ewes were assigned to four groups; a nicotine-exposed full-term and pre-term group, and control full-term and pre-term group. Lung tissue was collected for Western blot and IHC analysis of SP-A level, Western blot analysis of SP-D level and qPCR analysis of SP-A and SP-D mRNA expression. Exposure to nicotine significantly decreased SP-A gene expression (P = 0.01) and SP-A protein level in pre-term lambs. This finding suggests that maternal nicotine exposure during the last trimester of pregnancy alters a key component of lung innate immunity in offspring.

Nicotine

lung

lung surfactant proteins

fetal

Details

Title: Subtitle: Effects of nicotine on pulmonary surfactant proteins A and D in ovine lung epithelia
Creators: Tatjana Lazic - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa
Milan Matic - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa
Jack M Gallup - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa
Albert Van Geelen - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa
David K Meyerholz - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa
Branka Grubor - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa
Paula M Imerman - Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa
Marcia M.M.A de-Macedo - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa
Mark R Ackermann - Department of Veterinary Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa
Resource Type: Journal article
Publication Details: Pediatric pulmonology, Vol.45(3), pp.255-262
DOI: 10.1002/ppul.21153
PMID: 20131324
PMCID: PMC2981073
NLM abbreviation: Pediatr Pulmonol
ISSN: 8755-6863
eISSN: 1099-0496
Publisher: Wiley Subscription Services, Inc., A Wiley Company
Number of pages: 8
Language: English
Date published: 03/2010
Academic Unit: Pathology
Record Identifier: 9984083856802771

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