Effects of oxidative damage and telomerase activity on human articular cartilage chondrocyte senescence

James A Martin; Aloysius J Klingelhutz; Farid Moussavi-Harami; Joseph A Buckwalter

doi:10.1093/gerona/59.4.b324

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Effects of oxidative damage and telomerase activity on human articular cartilage chondrocyte senescence

Journal article

Open access

Peer reviewed

Effects of oxidative damage and telomerase activity on human articular cartilage chondrocyte senescence

James A Martin, Aloysius J Klingelhutz, Farid Moussavi-Harami and Joseph A Buckwalter

The journals of gerontology. Series A, Biological sciences and medical sciences, Vol.59(4), pp.324-B336

04/2004

DOI: 10.1093/gerona/59.4.b324

PMID: 15071075

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Abstract

Senescence compromises the ability of chondrocytes to maintain and repair articular cartilage. We hypothesized that oxidative stress and telomere loss contribute to chondrocyte senescence. To test this hypothesis, we compared the growth of human articular cartilage chondrocytes incubated in 5% O2 and 21% O2. Cells grown in 5% O2 reached 60 population doublings (PD) before senescing, but growth in 21% O2 induced DNA damage and premature senescence at less than 40 PD. Human telomerase reverse transcriptase (hTERT)-transduction failed to prevent chondrocyte senescence in 21% O2, but allowed 1 of 3 chondrocyte strains to exceed 90 PD in 5% O2. These results show that oxidative stress causes premature chondrocyte senescence. They may help explain the increased risk of osteoarthritis with age and after joint trauma and inflammation, and suggest that minimizing oxidative damage will help produce optimal results for chondrocyte transplantation.

Blotting, Southern

Chondrocytes - pathology

Mitosis

Humans

Osteoarthritis - metabolism

Mitochondria - pathology

beta-Galactosidase - analysis

Comet Assay

Telomere

Blotting, Western

Oxygen - metabolism

Aging - pathology

Cartilage, Articular - pathology

Analysis of Variance

Chondrocytes - physiology

Cartilage, Articular - metabolism

Telomerase - metabolism

Cell Culture Techniques

Osteoarthritis - pathology

Aging - metabolism

Details

Title: Subtitle: Effects of oxidative damage and telomerase activity on human articular cartilage chondrocyte senescence
Creators: James A Martin - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA 52242, USA
Aloysius J Klingelhutz
Farid Moussavi-Harami
Joseph A Buckwalter
Resource Type: Journal article
Publication Details: The journals of gerontology. Series A, Biological sciences and medical sciences, Vol.59(4), pp.324-B336
DOI: 10.1093/gerona/59.4.b324
PMID: 15071075
NLM abbreviation: J Gerontol A Biol Sci Med Sci
ISSN: 1079-5006
eISSN: 1758-535X
Publisher: United States
Grant note: P50 AR48939 / NIAMS NIH HHS
Language: English
Date published: 04/2004
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Microbiology and Immunology; Stead Family Department of Pediatrics; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation; Radiation Oncology; Injury Prevention Research Center
Record Identifier: 9984001121602771

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