Journal article
Effects of oxygen at high concentrations and food deprivation on cell division in lung alveoli of mice
Experimental and molecular pathology, Vol.26(3), pp.350-358
1977
DOI: 10.1016/0014-4800(77)90038-7
PMID: 862824
Abstract
Cell division is inhibited by exposure of mice to high oxygen (O 2). Because the exposed animals consume less food, relative to controls, a question arose about the effect of food deprivation per se on cell division. The purpose of this project was to compare the cell division in lung alveoli of animals: breathing air (A), exposed to high O 2 (O), deprived of food (D), and exposed to high O 2 plus deprived of food (OD). Swiss-Webster mice were divided into four equal groups: A, O, D, and OD. Animals from each group were sacrificed daily from 0–5 days. Cells synthesizing DNA and preparing to divide were labeled with tritiated thymidine. Labeled cells were visualized with autoradiography, counted with the light microscope, and expressed as a labeling index. Groups O and D showed statistically similar progressive decreases in cell labeling. Regression analysis testing for differences in trends showed that the combined data from groups O and D differed significantly from both the group A cell labeling, which did not decrease over 5 days, and the group OD cell labeling, which decreased dramatically. Generally similar results were found for body weight changes in the respective groups. These results show that: (1) Complete food deprivation with weight loss causes a decrease in the number of cells synthesizing DNA; (2) exposure to high O 2 causes a decrease in DNA synthesis, similar to that seen with food-deprivation weight loss; (3) exposure to high O 2 results in a decreased food intake and produces about the same weight loss as complete food deprivation; and (4) complete food deprivation of high-O 2-exposed mice produces more weight loss and decreases DNA synthesis even more. Thus, to the extent that it occurs, the decreased-food-associated weight loss during exposure to high O 2 is a principal factor in decreasing DNA synthesis.
Details
- Title: Subtitle
- Effects of oxygen at high concentrations and food deprivation on cell division in lung alveoli of mice
- Creators
- Jack D HackneyMichael J EvansRobert F BilsCharles E SpierMichael P Jones
- Resource Type
- Journal article
- Publication Details
- Experimental and molecular pathology, Vol.26(3), pp.350-358
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0014-4800(77)90038-7
- PMID
- 862824
- ISSN
- 0014-4800
- eISSN
- 1096-0945
- Language
- English
- Date published
- 1977
- Academic Unit
- Statistics and Actuarial Science; Biostatistics; Public Policy Center (Archive)
- Record Identifier
- 9983985916102771
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