Journal article
Effects of oxygen-glucose deprivation on microglial mobility and viability in developing mouse hippocampal tissues
Glia, Vol.60(11), pp.1747-1760
11/2012
DOI: 10.1002/glia.22394
PMCID: PMC3786781
PMID: 22847985
Abstract
As brain-resident immune cells, microglia (MG) survey the brain parenchyma to maintain homeostasis during development and following injury. Research in perinatal stroke, a leading cause of lifelong disability, has implicated MG as targets for therapeutic intervention during stroke. Although MG responses are complex, work in developing rodents suggests that MG limit brain damage after stroke. However, little is known about how energy-limiting conditions affect MG survival and mobility (motility and migration) in developing brain tissues. Here, we used confocal time-lapse imaging to monitor MG viability and mobility during hypoxia or oxygen-glucose deprivation (OGD) in hippocampal tissue slices derived from neonatal GFP-reporter mice (CX3CR1(GFP/+) ). We found that MG remain viable for at least 6 h of hypoxia but begin to die after 2 h of OGD, while both hypoxia and OGD reduce MG motility. Unexpectedly, some MG retain or recover motility during OGD and can engulf dead cells. Additionally, MG from younger neonates (P2-P3) are more resistant to OGD than those from older ones (P6-P7), indicating increasing vulnerability with developmental age. Finally, transient (2 h) OGD also increases MG death, and although motility is rapidly restored after transient OGD, it remains below control levels for many hours. Together, these results show that MG in neonatal mouse brain tissues are vulnerable to both transient and sustained OGD, and many MG die within hours after onset of OGD. Preventing MG death may, therefore, provide a strategy for promoting tissue restoration after stroke.
Details
- Title: Subtitle
- Effects of oxygen-glucose deprivation on microglial mobility and viability in developing mouse hippocampal tissues
- Creators
- Ukpong Eyo - Department of Biology, University of Iowa, Iowa City, Iowa, USAMichael E Dailey
- Resource Type
- Journal article
- Publication Details
- Glia, Vol.60(11), pp.1747-1760
- DOI
- 10.1002/glia.22394
- PMID
- 22847985
- PMCID
- PMC3786781
- NLM abbreviation
- Glia
- ISSN
- 0894-1491
- eISSN
- 1098-1136
- Publisher
- United States
- Grant note
- DC010362 / NIDCD NIH HHS R21 AA018823 / NIAAA NIH HHS S10 RR017941 / NCRR NIH HHS NS064006 / NINDS NIH HHS R01 NS043468 / NINDS NIH HHS P30 DC010362 / NIDCD NIH HHS
- Language
- English
- Date published
- 11/2012
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9983992062702771
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