Effects of staphylococcal toxic shock syndrome toxin 1 on aortic endothelial cells

Peter K Lee; Gregory M Vercellotti; James R Deringer; Patrick M Schlievert

doi:10.1093/infdis/164.4.711

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Effects of staphylococcal toxic shock syndrome toxin 1 on aortic endothelial cells

Journal article

Peer reviewed

Effects of staphylococcal toxic shock syndrome toxin 1 on aortic endothelial cells

Peter K Lee, Gregory M Vercellotti, James R Deringer and Patrick M Schlievert

The Journal of infectious diseases, Vol.164(4), pp.711-719

10/1991

DOI: 10.1093/infdis/164.4.711

PMID: 1654356

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Abstract

In staphylococcal toxic shock syndrome, hypotension and shock due to capillary leak may rapidly lead to death of the host. To investigate its pathogenesis, the cytotoxic effects of toxic shock syndrome toxin 1 (TSST-1) on porcine aortic endothelial cells (PAEC) were examined in vitro. TSST-1 killed PAEC (as measured by 51Cr release) in a dose- and time-dependent fashion and was blocked by anti-TSST-1 antibodies. Receptor-mediated endocytosis may be critical for the cytotoxic effects of TSST-1, as killing was inhibited by cold (4 degrees C) and by addition of chloroquine and methylamine. Furthermore, calcium and oxygen appeared necessary for TSST-1 effects on PAEC. Membrane receptor binding studies indicated PAEC bind TSST-1 with high affinity (Kd = 5.7 x 10(-7) M) and had 2.2 x 10(4) receptors/cell. Last, as measured by 125I-labeled albumin flux in a transendothelial permeability model, TSST-1 enhanced the permeability of PAEC monolayers in a dose- and time-dependent manner.

Endothelium, Vascular - cytology

Oxygen - pharmacology

Temperature

Enterotoxins - toxicity

Humans

Receptors, Cell Surface - analysis

Cell Membrane Permeability

Chloroquine - pharmacology

Bacterial Toxins

Swine

Receptors, Peptide

Methylamines - pharmacology

Staphylococcus aureus

Binding, Competitive

Calcium - pharmacology

Cells, Cultured

Receptors, Enterotoxin

Receptors, Guanylate Cyclase-Coupled

Animals

Guanylate Cyclase

Endothelium, Vascular - metabolism

Superantigens

Enterotoxins - metabolism

Aorta - cytology

Kinetics

Endotoxins - toxicity

Details

Title: Subtitle: Effects of staphylococcal toxic shock syndrome toxin 1 on aortic endothelial cells
Creators: Peter K Lee - Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455-0312
Gregory M Vercellotti
James R Deringer
Patrick M Schlievert
Resource Type: Journal article
Publication Details: The Journal of infectious diseases, Vol.164(4), pp.711-719
DOI: 10.1093/infdis/164.4.711
PMID: 1654356
NLM abbreviation: J Infect Dis
ISSN: 0022-1899
eISSN: 1537-6613
Publisher: United States
Grant note: CA-0913 / NCI NIH HHS HL-36611 / NHLBI NIH HHS HL-33793 / NHLBI NIH HHS
Language: English
Date published: 10/1991
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001110202771

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