Effects of the su(Hw) insulator protein on the expression of the divergently transcribed Drosophila yolk protein genes

K S Scott; P K Geyer

doi:10.1002/j.1460-2075.1995.tb00316.x

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Effects of the su(Hw) insulator protein on the expression of the divergently transcribed Drosophila yolk protein genes

Journal article

Open access

Peer reviewed

Effects of the su(Hw) insulator protein on the expression of the divergently transcribed Drosophila yolk protein genes

K S Scott and P K Geyer

The EMBO journal, Vol.14(24), pp.6258-6267

12/15/1995

DOI: 10.1002/j.1460-2075.1995.tb00316.x

PMCID: PMC394750

PMID: 8557045

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Abstract

The suppressor of Hairy-wing [su(Hw)] protein mediates the mutagenic effects of the gypsy retrotransposon by blocking enhancer activity. These repressive effects are general, can occur over long distances and require that the su(Hw) protein is bound between the affected enhancer and promoter. The effects of the su(Hw) binding region on yolk protein (yp) gene expression were determined. These genes are regulated by shared enhancers in the intergenic region, which provided a method to examine whether an enhancer blocked by the su(Hw) protein remained functional. We demonstrate that a blocked enhancer is completely active, supporting the proposal that the su(Hw) protein is an insulator protein that acts by forming a new boundary in a pre-existing chromatin domain, thereby preventing the interaction of regulatory elements located upstream of the insertion site with the promoter. In addition, we found that yp promoter function is not diminished by sharing enhancers, suggesting that these enhancers are not rate limiting for transcriptional activation. Lastly, our data indicate that yp promoter activity is interdependent, such that transcription from one promoter influences the level of activity of the linked promoter.

Repressor Proteins

Molecular Sequence Data

Male

DNA Primers - genetics

Tissue Distribution

Base Sequence

Cloning, Molecular

beta-Galactosidase - metabolism

Female

Transcription, Genetic

Nuclear Proteins - genetics

Drosophila - genetics

Alcohol Dehydrogenase - metabolism

Genes, Insect

Drosophila Proteins

Promoter Regions, Genetic

DNA-Binding Proteins - physiology

Retroelements

DNA-Binding Proteins - genetics

Animals

Enhancer Elements, Genetic

Models, Biological

Alcohol Dehydrogenase - genetics

Nuclear Proteins - physiology

Drosophila - metabolism

beta-Galactosidase - genetics

Egg Proteins - genetics

Details

Title: Subtitle: Effects of the su(Hw) insulator protein on the expression of the divergently transcribed Drosophila yolk protein genes
Creators: K S Scott - Department of Biochemistry, University of Iowa, College of Medicine, Iowa City 52242, USA
P K Geyer
Resource Type: Journal article
Publication Details: The EMBO journal, Vol.14(24), pp.6258-6267
Publisher: England
DOI: 10.1002/j.1460-2075.1995.tb00316.x
PMID: 8557045
PMCID: PMC394750
ISSN: 0261-4189
eISSN: 1460-2075
Grant note: GM42539 / NIGMS NIH HHS
Language: English
Date published: 12/15/1995
Academic Unit: Obstetrics and Gynecology; Biochemistry and Molecular Biology
Record Identifier: 9984025291802771

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