Journal article
Effects on dentin nanomechanical properties, cell viability and dentin wettability of a novel plant-derived biomodification monomer
Dental materials, Vol.40(10), pp.1584-1590
07/26/2024
DOI: 10.1016/j.dental.2024.07.010
PMID: 39068089
Abstract
To evaluate the effects of dentin biomodification agents (Proanthocyanidin (PAC), Cardol (CD) and Cardol-methacrylate (CDMA) on dentin hydrophilicity by contact angle measurement, viability of dental pulp stem cells (DPSCs) and nanomechanical properties of the hybrid layer (HL).
CDMA monomer was synthesized from cardol through methacrylic acid esterification. Human extracted third molars were used for all experiments. For nanomechanical tests, specimens were divided in four groups according to the primer solutions (CD, CDMA, PAC and control) were applied before adhesive and composite coating. Nanomechanical properties of the HL were analyzed by nanoindentation test using a Berkovich probe in a nanoindenter. Wettability test was performed on dentin surfaces after 1 min biomodification and measured by contact angle analysis. Cytotoxicity was assessed by a MTT assay with DPSCs after 48 and 72 h. Data were analyzed with Student's t test or Two-way ANOVA and Tukey HSD test (p < 0.05).
CD and CDMA solutions achieved greater hydrophobicity and increased the water-surface contact angles when compared to PAC and control groups (p < 0.05). PAC group showed a greater reduction of elastic modulus in nanoindentation experiments when compared to CD and CDMA groups (p < 0.05) after 4 months of aging. CD inhibited cell proliferation compared to all further materials (p < 0.05), whilst CDMA and PAC indicated no cell cytotoxicity to human DPSCs.
Cardol-methacrylate provided significantly higher hydrophobicity to dentin and demonstrated remarkable potential as collagen crosslinking, attaining the lowest decrease of HL’s mechanical properties. Furthermore, such monomer did not affect pulp cytotoxicity, thereby highlighting promising feasibility for clinical applications.
•Cell viability and dentin nanomechanical properties were surveyed for first time with collagen crosslinkers from CNSL.•Cardol and cardol-methacrylate as biomodification agents showed relevant reduction on dentin hydrophilicity.•Cardol and Cardol-methacrylate demonstrated promising reduction of hydrolytic degradation of the hybrid layer over time.•Cardol-methacrylate demonstrated no cell cytotoxicity in human DPSCs after 72 h.
Details
- Title: Subtitle
- Effects on dentin nanomechanical properties, cell viability and dentin wettability of a novel plant-derived biomodification monomer
- Creators
- Mário A. Moreira - Universidade Federal do CearáMadiana M. Moreira - Paulo Picanço School of DentistryDiego Lomonaco - Universidade Federal do CearáEduardo Cáceres - Universidad Andrés BelloLukasz Witek - New York UniversityPaulo G. Coelho - University of MiamiEmi Shimizu - Rutgers, The State University of New JerseyAngela Quispe-Salcedo - Niigata UniversityVictor P. Feitosa - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Dental materials, Vol.40(10), pp.1584-1590
- Publisher
- Elsevier Inc; London
- DOI
- 10.1016/j.dental.2024.07.010
- PMID
- 39068089
- ISSN
- 0109-5641
- eISSN
- 1879-0097
- Grant note
Duarte, at that time as Clinical Associate Professor of the Department of Basic Sciences and Craniofacial Biology at New York University College of Dentistry, for her valuable guidance, mentorship, and support throughout this research project. We also would like to acknowledge the support of Biomaterials Division of NYU, New York, NY, USA. The au-thors have no financial interest in any of the products used in this study.r Duarte, at that time as Clinical Associate Professor of the Department of Basic Sciences and Craniofacial Biology at New York University College of Dentistry, for her valuable guidance, mentorship, and support throughout this research project. We also would like to acknowledge the support of Biomaterials Division of NYU, New York, NY, USA. The au-thors have no financial interest in any of the products used in this study.
- Language
- English
- Electronic publication date
- 07/26/2024
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984693977402771
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