Journal article
Efficacy of Amphotericin B in Corneal Preservation Media After Extended Frozen Storage
Cornea, Vol.39(10), pp.1274-1277
10/2020
DOI: 10.1097/ICO.0000000000002381
PMID: 32482961
Abstract
To investigate the antimycotic activity of amphotericin B deoxycholate that has been previously frozen for 28 days before supplementation of Optisol-GS.
Triplicate Optisol-GS samples were inoculated with 10 colony-forming units (CFU) of Candida albicans. Each set of triplicate cultures was supplemented with 2.5 μg/mL of amphotericin B that was either freshly resuspended and never frozen, frozen overnight at -20°C and thawed, or frozen at -20°C for 4 weeks and thawed. The cultures were stored at 4°C, with aliquots taken at 0, 6, 24, and 72 hours for quantification. The efficacy of each preparation of amphotericin B in reducing C. albicans growth was assessed at these time points.
Six hours after antifungal supplementation, there was a 1.33 log10 CFU reduction with freshly resuspended amphotericin B, compared with a 1.31 log10 reduction with amphotericin B that was frozen overnight (P = 0.20) and a 1.18 log10 reduction with amphotericin B that was frozen for 4 weeks (P = 0.05). After 72 hours, there was a 2.72 log10 CFU reduction with freshly resuspended amphotericin B, a 2.64 log10 CFU reduction with amphotericin B that was frozen overnight (P = 0.45), and a 2.18 log10 CFU reduction with amphotericin B that was frozen for 4 weeks (P = 0.05).
Previously frozen amphotericin B remains highly effective against C. albicans. Optisol-GS supplemented with 2.5 μg/mL amphotericin B that was frozen for 4 weeks at -20°C resulted in >90% CFU reduction by 6 hours and >99% reduction by 72 hours.
Details
- Title: Subtitle
- Efficacy of Amphotericin B in Corneal Preservation Media After Extended Frozen Storage
- Creators
- Christopher S Sales - Department of Ophthalmology, Weill Cornell Medical College, New York, NYDoowon Huh - Department of Ophthalmology, Weill Cornell Medical College, New York, NYKhoa D Tran - Lions VisionGift, Portland, ORMegan M W Straiko - Lions VisionGift, Portland, ORMatthew W McCarthy - Department of Medicine, Weill Cornell Medical College, New York, NYAngela S Loo - Department of Pharmacy, Weill Cornell Medical College, New York, NY; andThomas J Walsh - Departments of Medicine, Pediatrics, and Microbiology and Immunology, Weill Cornell Medical College, New York, NY
- Resource Type
- Journal article
- Publication Details
- Cornea, Vol.39(10), pp.1274-1277
- DOI
- 10.1097/ICO.0000000000002381
- PMID
- 32482961
- ISSN
- 0277-3740
- eISSN
- 1536-4798
- Language
- English
- Date published
- 10/2020
- Academic Unit
- Ophthalmology and Visual Sciences
- Record Identifier
- 9984111183602771
Metrics
12 Record Views