Efficacy of Supplementary Irrigation Methods Against Bacterial Biofilm-Infected Root Canals Prepared With Minimally Invasive and Conventional Techniques

Giuliana Soimu; Abhishek Parolia; Anelise V Masiero; Fang Qian; Thomas Moninger; Jeffrey A Banas; Fabricio B Teixeira

doi:10.1111/aej.70024

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Efficacy of Supplementary Irrigation Methods Against Bacterial Biofilm-Infected Root Canals Prepared With Minimally Invasive and Conventional Techniques

Journal article

Peer reviewed

Efficacy of Supplementary Irrigation Methods Against Bacterial Biofilm-Infected Root Canals Prepared With Minimally Invasive and Conventional Techniques

Giuliana Soimu, Abhishek Parolia, Anelise V Masiero, Fang Qian, Thomas Moninger, Jeffrey A Banas and Fabricio B Teixeira

Australian endodontic journal, Vol.52(1), pp.46-58

04/2026

DOI: 10.1111/aej.70024

PMID: 41055238

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Abstract

This study evaluated the effectiveness of the GentleWave system (GWS), laser-activated irrigation (LAI), ultrasonic-activated irrigation (UAI) and sonic irrigation (SI) in removing a three-species biofilm from infected root canals prepared using minimally invasive techniques (MIT) and conventional instrumentation techniques (CIT). One hundred and ten single-canalled mandibular premolars were infected with the biofilm and assigned to five groups based on the supplementary irrigation method used. Biofilm removal was assessed using confocal laser scanning microscopy and scanning electron microscopy. In the CIT group, GWS resulted in a significantly higher proportion of dead cells compared to UAI and SI (p < 0.05), with no significant difference between GWS and LAI. In the MIT group, no significant differences were observed among the irrigation methods (p > 0.05). Although none of the approaches completely eliminated the biofilm, GWS and LAI were more effective than UAI and SI.

Biofilms

lasers

root canal irrigants

root canal therapy

GentleWave system

Details

Title: Subtitle: Efficacy of Supplementary Irrigation Methods Against Bacterial Biofilm-Infected Root Canals Prepared With Minimally Invasive and Conventional Techniques
Creators: Giuliana Soimu - Universidade Unigranrio
Abhishek Parolia - University of Iowa
Anelise V Masiero - University of Iowa
Fang Qian - University of Iowa
Thomas Moninger - University of Iowa Stead Family Children’s Hospital
Jeffrey A Banas - University of Iowa
Fabricio B Teixeira - The University of Texas at Dallas
Resource Type: Journal article
Publication Details: Australian endodontic journal, Vol.52(1), pp.46-58
DOI: 10.1111/aej.70024
PMID: 41055238
NLM abbreviation: Aust Endod J
ISSN: 1747-4477
eISSN: 1747-4477
Publisher: Wiley
Grant note: 202404307 / Univeristy of Iowa College of Dentsitry
Language: English
Electronic publication date: 10/07/2025
Date published: 04/2026
Academic Unit: Preventive and Community Dentistry; Endodontics; Pediatric Dentistry; Dental Research
Record Identifier: 9984968905302771

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