Journal article
Efficacy of microbial identification system for epidemiologic typing of coagulase-negative staphylococci
Journal of clinical microbiology, Vol.32(9), pp.2113-2119
09/1994
DOI: 10.1128/jcm.32.9.2113-2119.1994
PMCID: PMC263952
PMID: 7814534
Abstract
The lack of an adequate typing system hampers our understanding of the epidemiology of infections caused by coagulase-negative staphylococci (CoNS). CoNS have become recognized as important nosocomial pathogens and the principal cause of infections associated with invasive devices. Sensitive, specific, and convenient methods are needed to evaluate whether implementing infection control guidelines reduces the risk of nosocomial infections from CoNS and other pathogens. The Microbial Identification System (MIDI) (Microbial ID Inc., Newark, Del.), a semiautomated system for fatty acid methyl ester analysis, shows considerable promise for clinical and epidemiologic applications. Its predictive accuracy and reliability were tested by using epidemiologically related and replicated CoNS isolates as well as CoNS from epidemiologically unrelated clinical infections, which were obtained from five established hospital culture collections in diverse geographic locations. Two hundred isolates were fully characterized in 5 days by one person using MIDI, and the results were similar to those produced by more expensive and time-consuming conventional typing methods. MIDI appears to be a useful screening tool that could be used before more expensive and labor-intensive molecular methods. It offers important advantages to hospital epidemiologists and clinical microbiologists who must identify and type CoNS isolates.
Details
- Title: Subtitle
- Efficacy of microbial identification system for epidemiologic typing of coagulase-negative staphylococci
- Creators
- David Birnbaum - Department of Health Care and Epidemiology, University of British Columbia, Vancouver, CanadaLoreen Herwaldt - Department of Health Care and Epidemiology, University of British Columbia, Vancouver, CanadaDonald E Low - Department of Health Care and Epidemiology, University of British Columbia, Vancouver, CanadaMichael Noble - Department of Health Care and Epidemiology, University of British Columbia, Vancouver, CanadaMichael Pfaller - Department of Health Care and Epidemiology, University of British Columbia, Vancouver, CanadaRobert Sherertz - Department of Health Care and Epidemiology, University of British Columbia, Vancouver, CanadaAnthony W Chow - Department of Health Care and Epidemiology, University of British Columbia, Vancouver, Canada
- Resource Type
- Journal article
- Publication Details
- Journal of clinical microbiology, Vol.32(9), pp.2113-2119
- DOI
- 10.1128/jcm.32.9.2113-2119.1994
- PMID
- 7814534
- PMCID
- PMC263952
- ISSN
- 0095-1137
- eISSN
- 1098-660X
- Language
- English
- Date published
- 09/1994
- Academic Unit
- Infectious Diseases; Epidemiology; Pathology; Internal Medicine
- Record Identifier
- 9984094743202771
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