Efficient method to create integration-free, virus-free, Myc and Lin28-free human induced pluripotent stem cells from adherent cells

Anant Kamath; Sara Ternes; Stephen McGowan; Anthony English; Rama Mallampalli; Alan B Moy

doi:10.4155/fsoa-2017-0028

Back

Efficient method to create integration-free, virus-free, Myc and Lin28-free human induced pluripotent stem cells from adherent cells

Journal article

Open access

Peer reviewed

Efficient method to create integration-free, virus-free, Myc and Lin28-free human induced pluripotent stem cells from adherent cells

Anant Kamath, Sara Ternes, Stephen McGowan, Anthony English, Rama Mallampalli and Alan B Moy

Future science OA, Vol.3(3), pp.FSO211-FSO211

05/12/2017

DOI: 10.4155/fsoa-2017-0028

PMCID: PMC5583652

PMID: 28884008

Files and links (1)

url

https://doi.org/10.4155/fsoa-2017-0028View

Published (Version of record) Open Access

Abstract

Induced pluripotent stem cells (IPSCs) avoid the ethical controversy of embryonic stem cells (ESCs), but carry the same neoplastic risk of embryonic stem cell because of two deployed oncogenes ( c-Myc and Lin28 ). Pioneering work by Dr. Yamanaka showed that IPSC could be uncoupled from the neoplastic propensity by eliminating c-Myc . However, an oncogene-free and virus-free method has not been available. This report describes the first virus-free and oncogene-free IPSC method that efficiently produces pluripotent stem cells from adherent cells by the addition of reprogramming molecules. This approach satisfies an important regulatory pathway for developing safer future IPSC cell therapies.

episomal

IPS cell

reprogramming

Details

Title: Subtitle: Efficient method to create integration-free, virus-free, Myc and Lin28-free human induced pluripotent stem cells from adherent cells
Creators: Anant Kamath - Cellular Engineering Technologies (United States)
Sara Ternes - John Paul II Medical Research Institute
Stephen McGowan - Roy J. and Lucille A. Carver College of Medicine
Anthony English - Western New England University
Rama Mallampalli - University of Pittsburgh Medical Center
Alan B Moy - University of Iowa
Resource Type: Journal article
Publication Details: Future science OA, Vol.3(3), pp.FSO211-FSO211
DOI: 10.4155/fsoa-2017-0028
PMID: 28884008
PMCID: PMC5583652
NLM abbreviation: Future Sci OA
ISSN: 2056-5623
eISSN: 2056-5623
Publisher: Future Science Ltd
Alternative title: Kamath, Ternes, Mcgowan, English, Mallampalli & Moy
Language: English
Date published: 05/12/2017
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pulmonary, Critical Care, and Occupational Medicine; International Programs; Internal Medicine
Record Identifier: 9984359873402771

Metrics

7 Record Views

9 Times Cited - Web of Science

See more details