Efficient recovery of fluoroquinolone-susceptible and fluoroquinolone-resistant Escherichia coli strains from frozen samples

Ebbing Lautenbach; Evelyn Santana; Abby Lee; Pam Tolomeo; Nicole Black; Andrew Babson; Eli N Perencevich; Anthony D Harris; Catherine A Smith; Joel Maslow

doi:10.1086/529590

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Efficient recovery of fluoroquinolone-susceptible and fluoroquinolone-resistant Escherichia coli strains from frozen samples

Journal article

Peer reviewed

Efficient recovery of fluoroquinolone-susceptible and fluoroquinolone-resistant Escherichia coli strains from frozen samples

Ebbing Lautenbach, Evelyn Santana, Abby Lee, Pam Tolomeo, Nicole Black, Andrew Babson, Eli N Perencevich, Anthony D Harris, Catherine A Smith and Joel Maslow

Infection control and hospital epidemiology, Vol.29(4), pp.367-369

04/2008

DOI: 10.1086/529590

PMCID: PMC2892868

PMID: 18279070

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http://doi.org/10.1086/529590View

Open Access

Abstract

We assessed the rate of recovery of fluoroquinolone-resistant and fluoroquinolone-susceptible Escherichia coli isolates from culture of frozen perirectal swab samples compared with the results for culture of the same specimen before freezing. Recovery rates for these 2 classes of E. coli were 91% and 83%, respectively. The majority of distinct strains recovered from the initial sample were also recovered from the frozen sample. The strains that were not recovered were typically present only in low numbers in the initial sample. These findings emphasize the utility of frozen surveillance samples.

Rectum - microbiology

Escherichia coli - drug effects

Humans

Middle Aged

Drug Resistance, Bacterial

Male

Electrophoresis, Gel, Pulsed-Field

Freezing

Pennsylvania

Feces - microbiology

Cryopreservation

Escherichia coli - isolation & purification

Fluoroquinolones - pharmacology

Sensitivity and Specificity

Aged, 80 and over

Female

Aged

Anti-Bacterial Agents - pharmacology

Details

Title: Subtitle: Efficient recovery of fluoroquinolone-susceptible and fluoroquinolone-resistant Escherichia coli strains from frozen samples
Creators: Ebbing Lautenbach - Division of Infectious Diseases of the Department of Medicine, the Department of Biostatistics and Epidemiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104-6021, USA. ebbing@mail.med.upenn.edu
Evelyn Santana
Abby Lee
Pam Tolomeo
Nicole Black
Andrew Babson
Eli N Perencevich
Anthony D Harris
Catherine A Smith
Joel Maslow
Resource Type: Journal article
Publication Details: Infection control and hospital epidemiology, Vol.29(4), pp.367-369
DOI: 10.1086/529590
PMID: 18279070
PMCID: PMC2892868
NLM abbreviation: Infect Control Hosp Epidemiol
ISSN: 0899-823X
eISSN: 1559-6834
Publisher: United States
Grant note: R01-AG023792 / NIA NIH HHS U18 HS010399 / AHRQ HHS R01 AG023792 / NIA NIH HHS R01 AG023792-01A1 / NIA NIH HHS
Language: English
Date published: 04/2008
Academic Unit: Epidemiology; Internal Medicine
Record Identifier: 9983779295602771

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