Journal article
Efficient term development of vitrified ferret embryos using a novel pipette chamber technique
Biology of reproduction, Vol.79(5), pp.832-840
11/2008
DOI: 10.1095/biolreprod.107.067371
PMCID: PMC2658816
PMID: 18633142
Abstract
Development of an efficient cryopreservation technique for the domestic ferret is key for the long-term maintenance of valuable genetic specimens of this species and for the conservation of related endangered species. Unfortunately, current cryopreservation procedures, such as slow-rate freezing and vitrification with open pulled straws, are inefficient. In this report, we describe a pipette tip-based vitrification method that significantly improves the development of thawed ferret embryos following embryo transfer (ET). Ferret embryos at the morula (MR), compact morula (CM), and early blastocyst (EB) stages were vitrified using an Eppendorf microloader pipette tip as the chamber vessel. The rate of in vitro development was significantly (P < 0.05) higher among embryos vitrified at the CM (93.6%) and EB (100%) stages relative to those vitrified at the MR stages (58.7%). No significant developmental differences were observed when comparing CM and EB vitrified embryos with nonvitrified control CM (100%) and EB (100%) embryos. In addition, few differences in the ultrastructure of intracellular lipid droplets or in microfilament structure were observed between control embryos and embryos vitrified at any developmental stage. Vitrified-thawed CM/EB embryos cultured for 2 or 16 h before ET resulted in live birth rates of 71.3% and 77.4%, respectively. These rates were not significantly different from the control live birth rate (79.2%). However, culture for 32 h (25%) or 48 h (7.8%) after vitrification significantly reduced the rate of live births. These data indicate that the pipette chamber vitrification technique significantly improves the live birth rate of transferred ferret embryos relative to current state-of-the-art methods.
Details
- Title: Subtitle
- Efficient term development of vitrified ferret embryos using a novel pipette chamber technique
- Creators
- Xingshen Sun - Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City, Iowa 52242, USAZiyi LiYaling YiJuan ChenGregory H LenoJohn F Engelhardt
- Resource Type
- Journal article
- Publication Details
- Biology of reproduction, Vol.79(5), pp.832-840
- Publisher
- United States
- DOI
- 10.1095/biolreprod.107.067371
- PMID
- 18633142
- PMCID
- PMC2658816
- ISSN
- 0006-3363
- eISSN
- 1529-7268
- Grant note
- HL61234 / NHLBI NIH HHS P50 HL061234 / NHLBI NIH HHS R37 DK047967-20 / NIDDK NIH HHS R37 DK047967 / NIDDK NIH HHS P50 HL061234-08 / NHLBI NIH HHS R01 DK047967 / NIDDK NIH HHS P30 DK054759 / NIDDK NIH HHS R01 DK051315 / NIDDK NIH HHS DK47967 / NIDDK NIH HHS
- Language
- English
- Date published
- 11/2008
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025468902771
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