Journal article
Egress of Light Particles among Filopodia on the Surface of Varicella-Zoster Virus-Infected Cells
Journal of virology, Vol.82(6), pp.2821-2835
03/2008
DOI: 10.1128/JVI.01821-07
PMCID: PMC2258984
PMID: 18184710
Abstract
Varicella-zoster virus (VZV) is renowned for its very low titer when grown in cultured cells. There remains no single explanation for the low infectivity. In this study, viral particles on the surfaces of infected cells were examined by several imaging technologies. Few surface particles were detected at 48 h postinfection (hpi), but numerous particles were observed at 72 and 96 hpi. At 72 hpi, 75% of the particles resembled light (L) particles, i.e., envelopes without capsids. By 96 hpi, 85% of all particles resembled L particles. Subsequently, the envelopes of complete virions and L particles were investigated to determine their glycoprotein constituents. Glycoproteins gE, gI, and gB were detected in the envelopes of both types of particles in similar numbers; i.e., there appeared to be no difference in the glycoprotein content of the L particles. The viral particles emerged onto the cell surface amid actin-based filopodia, which were present in abundance within viral highways. Viral particles were easily detected at the base of and along the exterior surfaces of the filopodia. VZV particles were not detected within filopodia. In short, these results demonstrate that VZV infection of cultured cells produces a larger proportion of aberrant coreless particles than has been seen with any other previously examined alphaherpesvirus. Further, these results suggested a major disassociation between capsid formation and envelopment as an explanation for the invariably low VZV titer in cultured cells.
Details
- Title: Subtitle
- Egress of Light Particles among Filopodia on the Surface of Varicella-Zoster Virus-Infected Cells
- Creators
- John E Carpenter - Department of Pediatrics and Graduate Interdisciplinary Program, University of Iowa, Iowa City, Iowa 52242Jennifer A Hutchinson - Department of Pediatrics and Graduate Interdisciplinary Program, University of Iowa, Iowa City, Iowa 52242Wallen Jackson - Department of Pediatrics and Graduate Interdisciplinary Program, University of Iowa, Iowa City, Iowa 52242Charles Grose - Department of Pediatrics and Graduate Interdisciplinary Program, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.82(6), pp.2821-2835
- DOI
- 10.1128/JVI.01821-07
- PMID
- 18184710
- PMCID
- PMC2258984
- NLM abbreviation
- J Virol
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Publisher
- American Society for Microbiology (ASM)
- Language
- English
- Date published
- 03/2008
- Academic Unit
- Stead Family Department of Pediatrics; Nursing; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093338502771
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