Eicosapentaenoic acid metabolism in brain microvessel endothelium: effect on prostaglandin formation

N R Yerram; S A Moore; A A Spector

doi:10.1016/S0022-2275(20)38214-6

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Eicosapentaenoic acid metabolism in brain microvessel endothelium: effect on prostaglandin formation

Journal article

Open access

Peer reviewed

Eicosapentaenoic acid metabolism in brain microvessel endothelium: effect on prostaglandin formation

N R Yerram, S A Moore and A A Spector

Journal of lipid research, Vol.30(11), pp.1747-1757

11/1989

DOI: 10.1016/S0022-2275(20)38214-6

PMID: 2559136

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Abstract

Mouse brain microvessel endothelial cells convert eicosapentaenoic acid (EPA) to prostaglandin (PG) E3, PGI3, and several hydroxy fatty acid derivatives. Similar types of products are formed by these microvessel endothelial cells from arachidonic acid. The formation of PGI2 and PGE2 is reduced, however, when the brain microvessel endothelial cultures are incubated initially with EPA. Exposure to linolenic or docosahexaenoic acid also decreased the capacity of these microvessel endothelial cells to form PGI2 and PGE2, but the reductions were smaller than those produced by EPA. Like the endothelial cultures, intact mouse brain microvessels convert EPA into eicosanoids, and incubation with EPA reduces the subsequent capacity of the microvessels to produce PGI2 and PGE2. Brain microvessel endothelial cells took up less EPA than arachidonic acid, primarily due to lesser incorporation into the inositol, ethanolamine, and serine glycerophospholipids. By contrast, considerably more EPA than arachidonic acid was incorporated into triglycerides. These findings suggest that the microvessel endothelium may be a site of conversion of EPA to eicosanoids in the brain and that EPA availability can influence the amount of dienoic prostaglandins released by the brain microvasculature. Furthermore, the substantial incorporation of EPA into triglyceride suggests that this neutral lipid may play an important role in the processing and metabolism of EPA in brain microvessels.

Dinoprostone - biosynthesis

Epoprostenol - biosynthesis

Humans

Cells, Cultured

Eicosapentaenoic Acid - metabolism

Umbilical Veins

Endothelium, Vascular - drug effects

Chromatography, High Pressure Liquid

Calcimycin - pharmacology

Brain - blood supply

Microcirculation

Arachidonic Acids - metabolism

Animals

Eicosapentaenoic Acid - pharmacology

Endothelium, Vascular - metabolism

Mice

Arachidonic Acid

Fatty Acids - pharmacology

Details

Title: Subtitle: Eicosapentaenoic acid metabolism in brain microvessel endothelium: effect on prostaglandin formation
Creators: N R Yerram - Department of Biochemistry, University of Iowa, Iowa City 52242
S A Moore
A A Spector
Resource Type: Journal article
Publication Details: Journal of lipid research, Vol.30(11), pp.1747-1757
DOI: 10.1016/S0022-2275(20)38214-6
PMID: 2559136
NLM abbreviation: J Lipid Res
ISSN: 0022-2275
eISSN: 1539-7262
Publisher: United States
Grant note: NS-01096 / NINDS NIH HHS NS-24621 / NINDS NIH HHS HL-39308 / NHLBI NIH HHS
Language: English
Date published: 11/1989
Academic Unit: Pathology; Biochemistry and Molecular Biology; Internal Medicine
Record Identifier: 9984047883402771

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