Elastic Mineralized 3D Electrospun PCL Nanofibrous Scaffold for Drug Release and Bone Tissue Engineering

Jacob Miszuk; Zhipeng Liang; Jue Hu; Hanna Sanyour; Zhongkui Hong; Hao Fong; Hongli Sun

doi:10.1021/acsabm.1c00134

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Elastic Mineralized 3D Electrospun PCL Nanofibrous Scaffold for Drug Release and Bone Tissue Engineering

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Elastic Mineralized 3D Electrospun PCL Nanofibrous Scaffold for Drug Release and Bone Tissue Engineering

Jacob Miszuk, Zhipeng Liang, Jue Hu, Hanna Sanyour, Zhongkui Hong, Hao Fong and Hongli Sun

ACS applied bio materials, Vol.4(4), pp.3639-3648

04/19/2021

DOI: 10.1021/acsabm.1c00134

PMCID: PMC8103657

PMID: 33969280

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https://www.ncbi.nlm.nih.gov/pmc/articles/8103657View

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Abstract

Complex-shaped and critical-sized bone defects have been a clinical challenge for many years. Scaffold-based strategies such as hydrogels provide localized drug release while filling complex defect shapes, but ultimately possess weaknesses in low mechanical strength alongside a lack of macroporous and collagen-mimicking nanofibrous (NF) structures. Thus, there is a demand for mechanically strong, extracellular matrix (ECM) mimicking scaffolds that can robustly fit complex-shaped critical-sized defects and simultaneously provide localized, sustained, multiple growth factor release. We therefore developed a composite, biphasic polycaprolactone (PCL)/hydroxyapatite (HA) three-dimensional (3D)-nanofibrous (NF) scaffold for bone tissue regeneration using our innovative electrospun-based thermally induced self-agglomeration (TISA) technique. One intriguing feature of our ECM-mimicking TISA scaffolds is that they are highly elastic and porous even after evenly coated with minerals and can easily be pressed to fit different defect shapes. Furthermore, the biomimetic mineral deposition technique allowed us to simultaneously encapsulate different types of drugs, e.g., proteins and small molecules, on TISA scaffolds under physiologically mild conditions. Compared to scaffolds with physically surface-adsorbed phenamil, a BMP2 signaling agonist, incorporated phenamil composite scaffolds indicated less burst release and longer-lasting sustained release of phenamil with subsequently improved osteogenic differentiation of cells in vitro. Overall, our study indicated that the innovative press-fit 3D NF composite scaffold may be a robust tool for multiple-drug delivery and bone tissue engineering.

3D electrospun nanofibrous scaffold

drug release

press-fit

composite scaffold

bone tissue engineering

Details

Title: Subtitle: Elastic Mineralized 3D Electrospun PCL Nanofibrous Scaffold for Drug Release and Bone Tissue Engineering
Creators: Jacob Miszuk - University of Iowa
Zhipeng Liang - South Dakota School of Mines and Technology
Jue Hu - University of Iowa
Hanna Sanyour - University of South Dakota
Zhongkui Hong - University of South Dakota
Hao Fong - South Dakota School of Mines and Technology
Hongli Sun - University of Iowa
Resource Type: Journal article
Publication Details: ACS applied bio materials, Vol.4(4), pp.3639-3648
DOI: 10.1021/acsabm.1c00134
PMID: 33969280
PMCID: PMC8103657
NLM abbreviation: ACS Appl Bio Mater
ISSN: 2576-6422
eISSN: 2576-6422
Publisher: American Chemical Society
Grant note: DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research, award: R01DE029159; DOI: 10.13039/100000002, name: National Institutes of Health, award: T90DE023520; DOI: 10.13039/100011411, name: University of South Dakota; DOI: 10.13039/100000001, name: National Science Foundation, award: IIA-1335423
Language: English
Date published: 04/19/2021
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Dental Research
Record Identifier: 9984367645702771

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