Journal article
Electrical Forces Determine Glomerular Permeability
Journal of the American Society of Nephrology, Vol.21(12), pp.2053-2058
12/01/2010
DOI: 10.1681/ASN.2010030303
PMCID: PMC3014018
PMID: 20947631
Abstract
There is ongoing controversy about the mechanisms that determine the characteristics of the glomerular filter. Here, we tested whether flow across the glomerular filter generates extracellular electrical potential differences, which could be an important determinant of glomerular filtration. In micropuncture experiments in Necturus maculosus, we measured a potential difference across the glomerular filtration barrier that was proportional to filtration pressure (-0.045 mV/10 cm H2O). The filtration-dependent potential was generated without temporal delay and was negative within Bowman's space. Perfusion with the cationic polymer protamine abolished the potential difference. We propose a mathematical model that considers the relative contributions of diffusion, convection, and electrophoretic effects on the total flux of albumin across the filter. According to this model, potential differences of -0.02 to -0.05 mV can induce electrophoretic effects that significantly influence the glomerular sieving coefficient of albumin. This model of glomerular filtration has the potential to provide a mechanistic theory, based on experimental data, about the filtration characteristics of the glomerular filtration barrier. It provides a unique approach to the microanatomy of the glomerulus, renal autoregulation, and the pathogenesis of proteinuria.
Details
- Title: Subtitle
- Electrical Forces Determine Glomerular Permeability
- Creators
- Ralf Hausmann - RWTH Aachen UniversityChristoph Kuppe - Rhein Westfal TH Aachen, Univ Hosp, Dept Nephrol & Clin Immunol, Aachen, GermanyHerbert Egger - Rhein Westfal TH Aachen, Ctr Computat Engn Sci, Aachen, GermanyFrank Schweda - University of RegensburgVolker Knecht - Max Planck Institute of Colloids and InterfacesMarlies Elger - Heidelberg UniversitySylvia Menzel - Rhein Westfal TH Aachen, Univ Hosp, Dept Nephrol & Clin Immunol, Aachen, GermanyDouglas Somers - Univ Iowa, Coll Med, Dept Internal Med, Iowa City, IA 52242 USAGerald Braun - Rhein Westfal TH Aachen, Univ Hosp, Dept Nephrol & Clin Immunol, Aachen, GermanyAstrid Fuss - Rhein Westfal TH Aachen, Univ Hosp, Dept Nephrol & Clin Immunol, Aachen, GermanySandra Uhlig - Rhein Westfal TH Aachen, Univ Hosp, Dept Nephrol & Clin Immunol, Aachen, GermanyWilhelm Kriz - Heidelberg UniversityGeorge Tanner - Indiana UniversityJuergen Floege - Rhein Westfal TH Aachen, Univ Hosp, Dept Nephrol & Clin Immunol, Aachen, GermanyMarcus J. Moeller - Universitätsklinikum Aachen
- Resource Type
- Journal article
- Publication Details
- Journal of the American Society of Nephrology, Vol.21(12), pp.2053-2058
- DOI
- 10.1681/ASN.2010030303
- PMID
- 20947631
- PMCID
- PMC3014018
- NLM abbreviation
- J Am Soc Nephrol
- ISSN
- 1046-6673
- eISSN
- 1533-3450
- Publisher
- Amer Soc Nephrology
- Number of pages
- 6
- Grant note
- TP17 SFB/Transregio 57 / German Research Foundation (DFG) NephCure Foundation ERG-OPEN Medical Faculty of the RWTH Aachen Excellence Initiative by the DFG
- Language
- English
- Date published
- 12/01/2010
- Academic Unit
- Nephrology; Internal Medicine
- Record Identifier
- 9984359786102771
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